SELECTIVE AND SENSITIVE IN-VITRO DETECTION METHOD FOR ALUMINUM PHTHALOCYANINE PHOTOSENSITIZERS, USING LIQUID-CHROMATOGRAPHY AND DIODE-LASER INDUCED FLUORESCENCE

被引：16

作者：

MANK, AJG

GOOIJER, C

LINGEMAN, H

VELTHORST, NH

BRINKMAN, UAT

机构：

[1] Free University, Department of General and Analytical Chemistry, Boelelaan 1083

来源：

ANALYTICA CHIMICA ACTA | 1994年 / 290卷 / 1-2期

关键词：

LIQUID CHROMATOGRAPHY; FLUOROMETRY; ALUMINUM PHTHALOCYAMINE PHOTOSENSITIZERS; DIODE-LASER INDUCED FLUORESCENCE; PHOTOSENSITIZERS;

D O I：

10.1016/0003-2670(94)80045-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A combination of conventional-size liquid chromatography (LC) and visible diode-laser induced fluorescence (DIO-LIF) detection has been applied for the detection of photosensitizers used in photodynamic therapy. Of these photosensitizers, a mixture of disulfonated aluminum phthalocyanines (AlPcS(2)) has been chosen as a model. First, the influence of pH and solvent on the spectroscopic properties of di- and tetrasulfonated aluminum phthalocyanine has been examined, indicating the presence of multi-step ionization equilibria and the formation of aggregates. Direct fluorescence detection of AlPcS(2) is found to be unsatisfactory. For biological samples, the introduction of LC showed clear advantages, especially for faeces extracts. A detection limit of 7 x 10(-12) M was found for AlPcS, (S/N = 3 for the major component) in both rat urine and faeces extracts using the DIO-LIF system, which is 20-fold more sensitive than a conventional fluorescence detection system, in combination with chromatography.

引用

页码：103 / 120

页数：18

共 37 条

[1] PHOTODYNAMIC THERAPY OF CHEMICALLY-INDUCED AND ULTRAVIOLET-B RADIATION-INDUCED MURINE SKIN PAPILLOMAS BY CHLOROALUMINUM PHTHALOCYANINE TETRASULFONATE [J].