INDUCTION OF HEME OXYGENASE-1 IN THE RETINA BY INTENSE VISIBLE-LIGHT - SUPPRESSION BY THE ANTIOXIDANT DIMETHYLTHIOUREA

被引：147

作者：

KUTTY, RK ^{[1
]}

KUTTY, G ^{[1
]}

WIGGERT, B ^{[1
]}

CHADER, GJ ^{[1
]}

DARROW, RM ^{[1
]}

ORGANISCIAK, DT ^{[1
]}

机构：

[1] WRIGHT STATE UNIV,DEPT BIOCHEM & MOLEC BIOL,DAYTON,OH 45435

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1995年 / 92卷 / 04期

关键词：

D O I：

10.1073/pnas.92.4.1177

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The effect of intense visible light (light damage) on the expression of heme oxygenase 1 (HO-1), a protein induced by oxidative stress, was investigated in the rat retina. A sensitive reverse transcription-PCR assay demonstrated the expression of mRNA for HO-1 as well as HO-2, the noninducible HO form, in the normal retina, As analyzed by Northern blotting, however, HO-1 mRNA was barely detectable under normal circumstances, After exposure to intense visible light, retinas had markedly higher HO-1 mRNA levels than unexposed controls, with increases up to 52- and 98-fold at 12 and 24 hr of exposure, respectively Intense light exposure also resulted in an increase in HO-1 protein, In contrast, no appreciable change in HO-2 mRNA or protein was observed, The increase in HO-1 message was more pronounced in rats previously reared in the dark than in those reared in a weak cyclic-light environment. A marked decrease from the high level of HO-1 mRNA induced by light insult was observed when the animals were allowed to recover in the dark for 24 hr after light exposure, Most important, treatment of animals with 1,3-dimethylthiourea, a synthetic antioxidant, prior to light exposure effectively blocked the increase in HO-1 mRNA, Thus, HO-1 is a sensitive marker for assessing light-induced insult in the retina, Since increased expression of HO-1 is thought to be a cellular defense against oxidative damage, its expression may play an important role in protecting the retina against tight damage.

引用

页码：1177 / 1181

页数：5

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