ELECTROSPRAY IONIZATION MASS-SPECTROMETRIC PEPTIDE-MAPPING - A RAPID, SENSITIVE TECHNIQUE FOR PROTEIN-STRUCTURE ANALYSIS

Electrospray ionization mass spectrometric peptide mapping is demonstrated to be a useful new technique for protein structure analysis. The procedure involves the digestion of the protein with trypsin and subsequent analysis of the total unfractionated digest by electrospray ionization mass spectrometry. The utility of the technique for investigating protein structure is illustrated by a peptide mapping analysis of human apolipoprotein AI (Mr = 28 kDa). The technique is rapid, sensitive, and requires no prior separation of the peptides. The discrimination effects observed in other mass spectrometric methods are less important in the present procedure. © 1990.