ABSENCE OF INSULINOTROPIC GLUCAGONLIKE PEPTIDE-I(7-37) RECEPTORS ON ISOLATED RAT-LIVER HEPATOCYTES

被引：41

作者：

BLACKMORE, PF

MOJSOV, S

EXTON, JH

HABENER, JF

机构：

[1] HARVARD UNIV, MASSACHUSETTS GEN HOSP,SCH MED, HOWARD HUGHES MED INST,MOLEC ENDOCRINOL LAB, BOSTON, MA 02114 USA

[2] VANDERBILT UNIV, MED CTR, SCH MED, HOWARD HUGHES MED INST, NASHVILLE, TN 37232 USA

来源：

FEBS LETTERS | 1991年 / 283卷 / 01期

关键词：

GLUCAGONLIKE PEPTIDE-I; HEPATOCYTE RECEPTOR; PANCREATIC B-CELL; INSULINOTROPIC; CYCLIC AMP;

D O I：

10.1016/0014-5793(91)80541-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of glucagon and the glucagon-like peptide GLP-1(7-37) were compared in rat liver hepatocytes. Glucagon elevated cAMP, elevated intracellular free calcium ([Ca2+]i), activated phosphorylase and stimulated gluconeogenesis, whereas GLP-1(7-37) was without effect on any of these parameters. GLP-1(7-37) did not block any of the actions of glucagon. The glucagon analog, des His1[Glu9] glucagon amide, was a partial agonist in liver, but also was an effective antagonist of glucagon actions in liver but not those of GLP-1(7-37) in islet B cells. It was concluded that in the rat, GLP-1(7-37) is a potent insulin secretagogue [1] but is without effect on liver.

引用

页码：7 / 10

页数：4

共 28 条

[1]

BLACKMORE PF, 1985, METHOD ENZYMOL, V109, P550

[2]

BLACKMORE PF, 1986, J BIOL CHEM, V261, P1056

[3]

BLACKMORE PF, 1989, METHOD ENZYMOL, V173, P534

[4]

CHAREST R, 1983, J BIOL CHEM, V258, P8769

[5] GLUCAGONLIKE PEPTIDE-I STIMULATES INSULIN GENE-EXPRESSION AND INCREASES CYCLIC-AMP LEVELS IN A RAT ISLET CELL-LINE [J].

DRUCKER, DJ ;

PHILIPPE, J ;

MOJSOV, S ;

CHICK, WL ;

HABENER, JF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (10) :3434-3438

[6] BETA-CELL LINES DERIVED FROM TRANSGENIC MICE EXPRESSING A HYBRID INSULIN GENE ONCOGENE [J].

EFRAT, S ;

LINDE, S ;

KOFOD, H ;

SPECTOR, D ;

DELANNOY, M ;

GRANT, S ;

HANAHAN, D ;

BAEKKESKOV, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (23) :9037-9041

[7]

FEHMANN HC, 1991, IN PRESS FEBS LETT

[8]

FEHMANN HC, 1991, IN PRESS ENDOCRINOLO

[9] GLUCAGON-LIKE PEPTIDE-I ANALOGS - EFFECTS ON INSULIN-SECRETION AND ADENOSINE-3',5'-MONOPHOSPHATE FORMATION [J].

GEFEL, D ;

HENDRICK, GK ;

MOJSOV, S ;

HABENER, J ;

WEIR, GC .

ENDOCRINOLOGY, 1990, 126 (04) :2164-2168

[10] GLUCAGONLIKE PEPTIDE-1 DOES NOT HAVE A ROLE IN HEPATIC CARBOHYDRATE-METABOLISM [J].

GHIGLIONE, M ;

BLAZQUEZ, E ;

UTTENTHAL, LO ;

DEDIEGO, JG ;

ALVAREZ, E ;

GEORGE, SK ;

BLOOM, SR .

DIABETOLOGIA, 1985, 28 (12) :920-921

← 1 2 3 →