INTEREST OF A SIMPLE MOBILE PHASE CONTAINING DIETHYLAMINE-ACETIC ACID FOR HPLC ASSAY OF NICOTINE AND COTININE

被引：8

作者：

ROCHE, D ^{[1
]}

TOURY, I ^{[1
]}

LEQUANG, NT ^{[1
]}

ROUSSEL, G ^{[1
]}

EKINDJIAN, OG ^{[1
]}

机构：

[1] HOP LAENNEC,CTR PREVENT MALAD RESP,CONSULTAT ANTITABAC,F-75340 PARIS 07,FRANCE

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1991年 / 14卷 / 15期

关键词：

D O I：

10.1080/01483919108049366

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a simple mobile phase containing a diethylamine/acetic acid mixture free of salts and ion-pairing compounds for the assay of nicotine and cotinine in biological media by means of high-performance liquid chromatography. The best composition was found to be methanol/acetonitrile/acetic acid/water (230/20/2/748 v/v) adjusted to pH 5.00 or 6.40 with diethylamine. The results were excellent when compared with those obtained from mobile phases containing the following buffers: monopotassium phosphate/phosphoric acid, dipotassium phosphate/citric acid and sodium acetate/acetic acid. We found that 1) its buffering power is similar to that of the others and 2) it reduces pump-head wear and column washing time. Various columns may be used with this mobile phase, including the C18, 5-mu-m Ultrasphere (TM)

引用

页码：2919 / 2936

页数：18

共 15 条

[1]

CASTRO A, 1986, RES COMMUN CHEM PATH, V51, P393

[2]

CORREA P, 1983, LANCET, V2, P595

[3] MEASUREMENT OF NICOTINE AND COTIDINE IN URINE WITH HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

GODIN, J ;

HELLIER, G .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1989, 488 (02) :487-491

[4] PREPARATION OF BUFFERS FOR USE IN ENZYME STUDIES [J].

GOMORI, G .

METHODS IN ENZYMOLOGY, 1955, 1 :138-146

[5]

HAINES CF, 1974, CLIN PHARMACOL THER, V16, P1083

[6]

HARIHARAN M, 1988, CLIN CHEM, V34, P724

[7]

HENNEKENS CH, 1985, JAMA-J AM MED ASSOC, V253, P3003

[8]

HORTSMANN M, 1985, J CHROMATOGR, V344, P391

[9]

LEQUANG NT, 1989, CLIN CHEM, V35, P1456

[10]

MACHACEK DA, 1986, CLIN CHEM, V32, P979

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