A HIGHLY CONSERVED PHENYLALANINE IN THE ALPHA,BETA-T-CELL RECEPTOR (TCR) CONSTANT-REGION DETERMINES THE INTEGRITY OF TCR/CD3 COMPLEXES

被引:22
作者
CASPARBAUGUIL, S
ARNAUD, J
HUCHENQ, A
HEIN, WR
GEISLER, C
RUBIN, B
机构
[1] CHU PURPAN,CIGH,IMMUNOL CELLULAIRE & MOLEC LAB,CNRS,UPR 8291,F-31300 TOULOUSE,FRANCE
[2] BASEL INST IMMUNOL,BASEL,SWITZERLAND
[3] UNIV COPENHAGEN,INST MED MICROBIOL & IMMUNOL,COPENHAGEN,DENMARK
关键词
D O I
10.1111/j.1365-3083.1994.tb03469.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
In the present study, we have investigated the importance of a phenylalanine (phe(195)) in the Tcr-C alpha region on Tcr-alpha/beta/CD3 membrane expression. An exchange of phe(195) with a tyrosine residue does not affect Tcr/CD3 membrane expression; however, exchange with aspartic acid, histidine or valine prohibit completely Tcr/CD3 membrane expression. This seems to be due to a lack of interaction between mutated Tcr-alpha,beta/CD3-gamma epsilon,delta epsilon complexes and zeta(2) homodimers. The Tcr-C alpha region around phe(195) seems together with the same region in the Tcr-C beta region to constitute an interaction site for zeta(2) homodimers. The presence of phe(195) on both Tcr-C alpha and Tcr-C beta causes high avidity interaction with zeta 2 homodimers, whereas his(195) in both Tcr-C gamma and Tcr-C delta results in an apparently lower avidity interaction with zeta(2) homodimers. It is suggested that the phe(195) region (on beta-strand F) and eventually adjacent aromatic amino acid residues on beta-strand B region may play an important role in Tcr-alpha,beta/CD3 membrane expression, in Tcr-alpha,beta/CD3 competition with Tcr-gamma,delta/CD3 complexes for zeta 2 homodimers and in the control of formation of 'mixed' Tcr heterodimers.
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页码:323 / 336
页数:14
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