OXIDATIVE STRESS IN GASTRIC-MUCOSAL INJURY - ROLE OF PLATELET-ACTIVATING FACTOR-ACTIVATED GRANULOCYTES

被引：9

作者：

FUKUMURA, D ^{[1
]}

KUROSE, I ^{[1
]}

MIURA, S ^{[1
]}

TSUCHIYA, M ^{[1
]}

ISHII, H ^{[1
]}

机构：

[1] KEIO UNIV,SCH MED,DEPT INTERNAL MED,SHINJUKU KU,TOKYO 160,JAPAN

来源：

JOURNAL OF GASTROENTEROLOGY | 1995年 / 30卷 / 05期

关键词：

DICHLOROFLUORESCEIN; OXYGEN RADICALS; MICROCIRCULATION;

D O I：

10.1007/BF02367780

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Temporal and spatial changes due to oxidative stress in the rat gastric mucosa were visualized and quantified during the process of mucosal hemorrhagic change. The fluorescence associated with dichlorofluorescein (DCF), a hydroperoxide-sensitive fluorochrome, increased 30 min after repeated electrical stimuli to the gastric artery. The increase in the fluorescence was enhanced in the area between two adjacent collecting venules. The content of platelet-activating factor (PAF), the activity of myeloperoxidase (MPO) in the gastric mucosa, the area of mucosal lesions, and the luminol-dependent chemiluminescence activity in zymosan-treated blood samples, obtained from the gastric vein, were measured and found to increase significantly 30 min after the stimuli. The intravenous injection of CV-6209, a PAF antagonist, 5 min prior to the stimuli significantly inhibited the DCF activation, the increases in PAF level and MPO activity, the mucosal hemorrhagic change, and the elevation in chemiluminescence activity. In addition, continuous infusion of superoxide dismutase also inhibited all these changes, except for chemiluminescence activity. These results suggest that oxygen radicals derived from PAF-activated granulocytes induce oxidative stress, and that oxidative changes are actually implicated in the pathogenesis of gastric mucosal injury.

引用

页码：565 / 571

页数：7

共 25 条

[1]

Granger D.N., Benoit J.N., Suzuki M., Et al., Leukocyte adherence to venular endothelium during ischemia-reperfusion, Am J Physiol, 257, pp. G683-G688, (1989)

[2]

Grisham M.B., Granger D.N., Neutrophil-mediated mucosal injury: Role of reactive oxygen metabolites, Digestive Diseases and Sciences, 33, pp. 6S-15S, (1988)

[3]

Kvietys P.R., Twohig B., Danzell J., Et al., Ethanol-induced injury to the rat gastric mucosa. Role of neutrophils and xanthine oxidase-derived radicals, Gastroenterology, 98, pp. 909-920, (1990)

[4]

Smith S.M., Holm-Rutili L., Perry M.A., Et al., Role of neutrophils in hemorrhagic shock-induced gastric mucosal injury in the rat, Gastroenterology, 93, pp. 466-471, (1988)

[5]

Vedder N.B., Winn R.K., Rice C.L., Et al., A monoclonal antibody to the adherence-promoting leukocyte glycoprotein, CD18, reduces organ injury and improves survival from hemorrhagic shock and resuscitation in rabbits, J Clin Invest, 81, pp. 939-944, (1988)

[6]

Suematsu M., Kurose I., Asako H., Et al., In vivo visualization of oxyradical-dependent photoemission during endothelium-granulocyte interaction in microvascular beds treated with platelet-activating factor, J Biochem, 106, pp. 355-361, (1989)

[7]

Suzuki M., Asako H., Kubes P., Et al., Neutrophil-derived oxidants promote leukocyte adherence in postcapillary venules, Microvasc Res, 42, pp. 125-138, (1991)

[8]

Itoh M., Guth P.H., Role of oxygen-derived free radicals in hemorrhagic shock-induced gastric lesions in the rat, Gastroenterology, 88, pp. 1162-1167, (1985)

[9]

Kurose I., Suematsu M., Miura S., Et al., Involvement of superoxide anion and platelet-activating factor in increased tissue-type plasminogen activator during rat gastric microvascular damage, Thromb Res, 62, pp. 241-248, (1991)

[10]

Kurose I., Miura S., Fukumura D., Et al., Role of platelet-activating factor in fibrinolytic activation in the pathogenesis of gastric mucosal damage induced by endothelin-1, Gut, 33, pp. 868-871, (1992)

← 1 2 3 →