COCULTURE CELLS THAT EXPRESS LEUKEMIA INHIBITORY FACTOR (LIF) ENHANCE MOUSE BLASTOCYST DEVELOPMENT IN-VITRO

被引：72

作者：

KAUMA, SW

MATT, DW

机构：

[1] Department of Obstetrics and Gynecology Medical College of Virginia, Virginia Commonwealth University, Richmond, 23298, Virginia

来源：

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS | 1995年 / 12卷 / 02期

关键词：

LEUKEMIA INHIBITORY FACTOR; BLASTOCYST; MURINE; COCULTURE;

D O I：

10.1007/BF02211386

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Purpose: Leukemia inhibitory factor is a cytokine that plays an important role in implantation and enhances mouse preimplantation embryo development in vitro. Since leukemia inhibitory factor enhances early embryo development, we tested the hypothesis that coculture cells that express leukemia inhibitory factor would enhance mouse blastocyst development in vitro. In this study, Northern analysis for leukemia inhibitory factor was performed an total RNA extracted from Vero cells, human embryonic fibroblasts, and human placental fibroblasts. Methods: Two-cell mouse embryos were cultured to blastocyst in Ham's F-10 with 15% human cord serum (control) or with the coculture cells. Northern analysis demonstrated expression of LIF mRNA in Vero cells and embryonic fibroblasts bur not in placental fibroblasts. Development to blastocyst was significantly enhanced in two-cell embryos cultured with Vero cells (86%, 140/163) and embryonic fibroblasts (83%, 118/142) when compared to controls (71%, 67/94, P < 0.05) or placental fibroblasts (71%, 95/134), P < 0.05). Conclusion: These data suggest that coculture cells that express leukemia inhibitory factor may be superior to nonleukemia inhibitory factor expressing cells for early preimplantation embryo development.

引用

页码：153 / 156

页数：4

共 14 条

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