BOVINE THECA AND GRANULOSA-CELL INTERACTIONS MODULATE THEIR GROWTH, MORPHOLOGY, AND FUNCTION

被引:46
作者
KOTSUJI, F
KAMITANI, N
GOTO, K
TOMINAGA, T
机构
[1] Department of OB/GYN, Fukui Medical School, Yoshida-Gun, Fukui 910-11, Shimoaizuki Matsuoka-Cho
关键词
D O I
10.1095/biolreprod43.5.726
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
We have developed a culture system in which bovine granulosa and theca cells are allowed to attach to opposite sides of a collagen membrane. We studied the interaction between theca and granulosa cells by investigating the morphology, proliferation, and steroidogenesis of the cells. Granulosa cells cultured alone were flattened and polygonal and formed monolayer sheets. Granulosa cells cocultured with theca cells formed multilayer sheets. The apical surface of each cell appeared convex. Numerous filopodia spread over the cellular surface connecting cells. Theca cells cultured alone were thin, flat, and spindle-shaped. Theca cells cocultured with granulosa cells were also spindle-shaped; however, the apical surface appeared convex. Cocultured cells were more densely packed than theca cells cultured alone. The number of both granulosa and theca cells in the cocultured group increased approximately twofold compared to control cells cultured alone. Progesterone content per 1 x 105 granulosa cells in 24-h culture medium of the cocultured group was reduced to 40% of that of the control group. In contrast, androstenedione content per 1 x 105 theca cells of the cocultured group increased approximately threefold compared to androstenedione content of control group. These results indicate that communication between these two types of follicular cells results in reciprocal modulation of their proliferation, morphology, and function.
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收藏
页码:726 / 732
页数:7
相关论文
共 13 条
[1]   INVIVO AND INVITRO EFFECTS OF ANDROGENS ON RAT OVARIAN GRANULOSA-CELLS [J].
ANDERSON, E .
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 1989, 160 (04) :782-788
[2]   ANDROGENS AUGMENT FSH-INDUCED PROGESTERONE SECRETION BY CULTURED RAT GRANULOSA-CELLS [J].
ARMSTRONG, DT ;
DORRINGTON, JH .
ENDOCRINOLOGY, 1976, 99 (05) :1411-1414
[3]   BOVINE THECAL CELLS SECRETE FACTOR(S) THAT PROMOTE GRANULOSA-CELL PROLIFERATION [J].
BENDELL, JJ ;
LOBB, DK ;
CHUMA, A ;
GYSLER, M ;
DORRINGTON, JH .
BIOLOGY OF REPRODUCTION, 1988, 38 (04) :790-797
[4]  
CHRAMBARD M, 1983, J CELL BIOL, V96, P1173
[5]  
CUNHA GR, 1983, RECENT PROG HORM RES, V39, P559
[6]   MAINTENANCE AND INDUCTION OF MORPHOLOGICAL-DIFFERENTIATION IN DISSOCIATED MAMMARY EPITHELIUM ON FLOATING COLLAGEN MEMBRANES [J].
EMERMAN, JT ;
PITELKA, DR .
IN VITRO-JOURNAL OF THE TISSUE CULTURE ASSOCIATION, 1977, 13 (05) :316-328
[7]   HORMONAL EFFECTS ON INTRACELLULAR AND SECRETED CASEIN IN CULTURES OF MOUSE MAMMARY EPITHELIAL-CELLS ON FLOATING COLLAGEN MEMBRANES [J].
EMERMAN, JT ;
ENAMI, J ;
PITELKA, DR ;
NANDI, S .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (10) :4466-4470
[8]   BOVINE THECA AND GRANULOSA-CELLS INTERACT TO PROMOTE ANDROGEN PRODUCTION [J].
FORTUNE, JE .
BIOLOGY OF REPRODUCTION, 1986, 35 (02) :292-299
[9]   STIMULATORY ACTION OF FOLLICLE-STIMULATING-HORMONE AND ANDROGENS ON THE RESPONSIVENESS OF RAT GRANULOSA-CELLS TO GONADOTROPINS INVITRO [J].
GOFF, AK ;
LEUNG, PCK ;
ARMSTRONG, DT .
ENDOCRINOLOGY, 1979, 104 (04) :1124-1129
[10]   POLARIZED SECRETION OF ANDROGEN-BINDING PROTEIN AND TRANSFERRIN BY SERTOLI CELLS GROWN IN A BICAMERAL CULTURE SYSTEM [J].
HADLEY, MA ;
DJAKIEW, D ;
BYERS, SW ;
DYM, M .
ENDOCRINOLOGY, 1987, 120 (03) :1097-1103