POTENTIOMETRIC AND SPECTROSCOPIC STUDIES OF THE CU(II) COMPLEXES OF ALA-ARG8-VASOPRESSIN AND OXYTOCIN - 2 VASOPRESSIN-LIKE PEPTIDES

The results are reported of a potentiometric and spectroscopic study of the H+ and Cu2+ Complexes of Ala-Arg8-vasopressin (Ala-AVP) and oxytocin at 25-degrees-C and an ionic strength of 0.10 mol dm-3 (KNO3). The coordination chemistry of oxytocin and Cu(II) has been shown to be virtually identical to that of Arg8-vasotocin, forming unusually stable complexes with four nitrogen coordination (4N complexes) below pH 7. Spectroscopic evidence suggests weak interaction between Cu(II) and the sulphur atom of the -Cys6- residue in the 2N species (pH congruent-to 6) but this is absent in the 4N complex. Evidence is also presented for perturbation of electronic transitions within the aromatic ring of the Tyr residue by Cu(II). While the physiological potency of Ala-AVP is very high, its coordination chemistry differs significantly from that of Arg8-vasopressin. With Cu(II) it forms complexes of similar stability to those with tetraglycine, demonstrating that the addition of an alanyl residue to the amino-terminal of the peptide destroys the conformation which is particularly favorable for rapid 4N coordination.