ALPHA(1)BETA(1) INTEGRIN ON NEURAL CREST CELLS RECOGNIZES SOME LAMININ SUBSTRATA IN A CA2+-INDEPENDENT MANNER

被引:60
作者
LALLIER, T
BRONNERFRASER, M
机构
[1] Developmental Biology Center, University of California, Irvine
关键词
D O I
10.1083/jcb.119.5.1335
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Neural crest cells migrate along pathways containing laminin and other extracellular matrix molecules. In the present study, we functionally and biochemically identify an alpha1beta1 integrin heterodimer which bears the HNK-1 epitope on neural crest cells. Using a quantitative cell adhesion assay, we find that this heterodimer mediates attachment to laminin substrata prepared in the presence of Ca2+. Interestingly, neural crest cells bind to laminin-Ca2+ substrata in the presence or absence of divalent cations in the cell attachment medium. In contrast, the attachment of neural crest cells to laminin substrata prepared in the presence of EDTA, heparin, Mg2+, or Mn2+ requires divalent cations. Interactions with these laminin substrata are mediated by a different integrin heterodimer, since antibodies against beta1 but not alpha1, integrins inhibit neural crest cell attachment. Thus, the type of laminin substratum appears to dictate the choice of laminin receptor used by neural crest cells. The laminin conformation is determined by the ratio of laminin to Ca2+, though incorporation of heparin during substratum polymerization alters the conformation even in the presence of Ca2+. Once polymerized, the substratum appears stable, not being altered by soaking in either EDTA or divalent cations. Our findings demonstrate: (a) that the alpha1beta1 integrin can bind to some forms of laminin in the absence of soluble divalent cations; (b) that substratum preparation conditions alter the conformation of laminin such that plating laminin in the presence of Ca2+ and/or heparin modulates its configuration; and (c) that neural crest cells utilize different integrins to recognize different laminin conformations.
引用
收藏
页码:1335 / 1345
页数:11
相关论文
共 89 条
[1]   HUMAN SMOOTH-MUSCLE VLA-1 INTEGRIN - PURIFICATION, SUBSTRATE-SPECIFICITY, LOCALIZATION IN AORTA, AND EXPRESSION DURING DEVELOPMENT [J].
BELKIN, VM ;
BELKIN, AM ;
KOTELIANSKY, VE .
JOURNAL OF CELL BIOLOGY, 1990, 111 (05) :2159-2170
[2]   TENASCIN MEDIATES CELL ATTACHMENT THROUGH AN RGD-DEPENDENT RECEPTOR [J].
BOURDON, MA ;
RUOSLAHTI, E .
JOURNAL OF CELL BIOLOGY, 1989, 108 (03) :1149-1155
[3]   PERTURBATION OF CRANIAL NEURAL CREST MIGRATION BY THE HNK-1 ANTIBODY [J].
BRONNERFRASER, M .
DEVELOPMENTAL BIOLOGY, 1987, 123 (02) :321-331
[4]   ANALYSIS OF THE EARLY STAGES OF TRUNK NEURAL CREST MIGRATION IN AVIAN EMBRYOS USING MONOCLONAL-ANTIBODY HNK-1 [J].
BRONNERFRASER, M .
DEVELOPMENTAL BIOLOGY, 1986, 115 (01) :44-55
[5]   AN ANTIBODY TO A RECEPTOR FOR FIBRONECTIN AND LAMININ PERTURBS CRANIAL NEURAL CREST DEVELOPMENT INVIVO [J].
BRONNERFRASER, M .
DEVELOPMENTAL BIOLOGY, 1986, 117 (02) :528-536
[6]   A MONOCLONAL-ANTIBODY AGAINST A LAMININ-HEPARAN SULFATE PROTEOGLYCAN COMPLEX PERTURBS CRANIAL NEURAL CREST MIGRATION INVIVO [J].
BRONNERFRASER, M ;
LALLIER, T .
JOURNAL OF CELL BIOLOGY, 1988, 106 (04) :1321-1329
[7]   ALTERATIONS IN NEURAL CREST MIGRATION BY A MONOCLONAL-ANTIBODY THAT AFFECTS CELL-ADHESION [J].
BRONNERFRASER, M .
JOURNAL OF CELL BIOLOGY, 1985, 101 (02) :610-617
[8]   DISTRIBUTION AND FUNCTION OF TENASCIN DURING CRANIAL NEURAL CREST DEVELOPMENT IN THE CHICK [J].
BRONNERFRASER, M .
JOURNAL OF NEUROSCIENCE RESEARCH, 1988, 21 (2-4) :135-147
[9]  
BUCK CA, 1987, J CELL SCI, P231
[10]   INTEGRIN (THE CSAT ANTIGEN) - FUNCTIONALITY REQUIRES OLIGOMERIC INTEGRITY [J].
BUCK, CA ;
SHEA, E ;
DUGGAN, K ;
HORWITZ, AF .
JOURNAL OF CELL BIOLOGY, 1986, 103 (06) :2421-2428