AMINOACYL-TRANSFER-RNA SYNTHETASE COMPLEX IN SACCHAROMYCES-CEREVISIAE

被引：12

作者：

HARRIS, CL

KOLANKO, CJ

机构：

[1] Department of Biochemistry, West Virginia University, Robert C Byrd Health Sciences Center, Morgantown, WV 26506-9142

来源：

BIOCHEMICAL JOURNAL | 1995年 / 309卷

关键词：

D O I：

10.1042/bj3090321

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The size distribution of aminoacyl-tRNA synthetase activity was investigated in cell extracts prepared from Saccharomyces cerevisiae. Bio-Gel A-5M chromatography of 105000 g supernatants separated isoleucyl-tRNA synthetase activity into three peaks, with apparent molecular masses (Da) of about 100000, 350000 and 10(6) or greater. Similar results were obtained with synthetases specific for glutamic acid, serine and tyrosine. Sucrose-density-gradient centrifugation of yeast supernatants also provided evidence for the existence of synthetase complexes. These data provide the first evidence for the existence of a high-molecular-mass aminoacyl-tRNA. synthetase complex in yeast, perhaps similar to those reported in higher eukaryotes.

引用

页码：321 / 324

页数：4

共 17 条

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