A RAPID AND SENSITIVE HEMOLYSIS NEUTRALIZATION ASSAY FOR PALYTOXIN

被引：147

作者：

BIGNAMI, GS

机构：

[1] Hawaii Biotechnology Group, Inc., Aiea, HI 96701

来源：

TOXICON | 1993年 / 31卷 / 06期

关键词：

D O I：

10.1016/0041-0101(93)90389-Z

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The hemolytic action of palytoxin was exploited to develop a simple, sensitive assay with specificity based on a palytoxin neutralizing monoclonal antibody. Suspensions of murine erythrocytes incubated at 37-degrees-C in round-bottom microtiter trays formed visible cell pellets which could be lysed by palytoxin. Hemolysis by palytoxin was time- and temperature-dependent, with a 24 hr detection limit of 1 pg/ml. The assay selectively detected palytoxin in a crude extract of Palythoa tuberculosa.

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页码：817 / 820

页数：4

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