ECTOGALACTOSYLTRANSFERASE STUDIES IN FIBROBLASTS AND CONCANAVALIN A STIMULATED LYMPHOCYTES

被引：9

作者：

PATT, LM

ENDRES, RO

LUCAS, DO

GRIMES, WJ

机构：

[1] UNIV ARIZONA, COLL MED, DEPT BIOCHEM, TUCSON, AZ 85724 USA

[2] UNIV ARIZONA, COLL MED, DEPT MICROBIOL, TUCSON, AZ 85724 USA

来源：

JOURNAL OF CELL BIOLOGY | 1976年 / 68卷 / 03期

关键词：

D O I：

10.1083/jcb.68.3.799

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Hydrolysis of the nucleotide sugar can apparently cause errors in the detection of an ectoglycosyltransferase. Spleen cell suspensions can incorporate radioactivity when incubated with labeled UDP-galactose, but all the activity is due to decomposition of the nucleotide sugar and uptake of the free sugar. The fibroblast cell lines (mouse Balb/c-3TC SV-40 transformed) can incorporate carbohydrate directly from UDP-galactose. Several criteria are presented which can be used to demonstrate that a nucleotide sugar is the direct carbohydrate donor.

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页码：799 / 802

页数：4

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