A NONINVASIVE TECHNIQUE FOR SEQUENTIAL COLLECTION OF EARTHWORM (LUMBRICUS-TERRESTRIS) LEUKOCYTES DURING SUBCHRONIC IMMUNOTOXICITY STUDIES

被引：206

作者：

EYAMBE, GS

GOVEN, AJ

FITZPATRICK, LC

VENABLES, BJ

COOPER, EL

机构：

[1] UNIV CALIF LOS ANGELES,SCH MED,DEPT ANAT & CELL BIOL,LOS ANGELES,CA 90024

[2] TRAC LABS,DENTON,TX 76201

来源：

LABORATORY ANIMALS | 1991年 / 25卷 / 01期

关键词：

EARTHWORMS; LUMBRICUS-TERRESTRIS; IMMUNE RESPONSES; LEUKOCYTES; CELOMOCYTES; CELOMOCYTE COLLECTION TECHNIQUES;

D O I：

10.1258/002367791780808095

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

We have compared two methods for collecting earthworm leukocytes (coelomocytes) with respect to cell yield, viability and behaviour in immunoassays. Non-invasive extrusion was more efficient than puncturing the coelomic cavity. Extrusion does not produce trauma to earthworms maintained under long term laboratory conditions. Neither technique modified immune assays, as determined by erythrocyte and secretory rosette formation, and phagocytosis, since all 3 were functionally equivalent. After an initial extrusion, sequential leukocyte collections by extrusion are possible at intervals of 6 weeks without affecting total and differential cell counts and rosette formation.

引用

页码：61 / 67

页数：7

共 21 条

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