DEVELOPMENT OF A NEW LIVE ATTENUATED MUMPS-VIRUS VACCINE IN HUMAN-DIPLOID CELLS

被引:19
作者
SASSANI, A [1 ]
MIRCHAMSY, H [1 ]
SHAFYI, A [1 ]
AHOURAI, P [1 ]
RAZAVI, J [1 ]
GHOLAMI, MR [1 ]
MOHAMMADI, A [1 ]
EZZI, A [1 ]
RAHMANI, M [1 ]
FATEH, G [1 ]
PARAVANDI, T [1 ]
机构
[1] RAZI STATE SERUM & VACCINE INST,PBO 11365,TEHRAN 1558,IRAN
关键词
D O I
10.1016/1045-1056(91)90036-J
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A new live attenuated mumps vaccine was developed in human diploid cells. The S-12 virus was isolated from a 10-year-old girl showing typical symptoms of mumps infection, the diagnosis was confirmed by a pediatrician. The virus was isolated in green monkey kidney cells, without passage in chick embryo cavity or chick embryo fibroblasts. Attenuation of the wild virus was performed by serial passages in human diploid cells (MRC-5). The attenuated virus was characterized by identity tests, as well as by a reduction in plaque size, as marker tests. The virus was free from adventitious agents and safe for laboratory animals as well as for monkeys. The reactogenicity and immunogenicity of the S-12 virus for man was investigated by administration of a monovalent vaccine to 20 seronegative adult male volunteers and 30 children aged 1 to 5 years without history of mumps infection or vaccination. Seroconversion was obtained in 95% of the vaccinees. The new vaccine has the advantage of not requiring specific pathogen-free eggs, and being free from avian proteins and therefore can be used in sensitized patients. © 1991.
引用
收藏
页码:203 / 211
页数:9
相关论文
共 26 条
  • [1] SENSITIVE HEMAGGLUTINATION INHIBITION TEST FOR MUMPS ANTIBODY
    ALBRECHT, P
    KLUTCH, M
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1981, 13 (05) : 870 - 876
  • [2] MUMPS VACCINE L-ZAGREB, PREPARED IN CHICK FIBROBLASTS .1. PRODUCTION AND FIELD TRIALS
    BECK, M
    WELSZMALECEK, R
    MESKOPREJAC, M
    RADMAN, V
    JUZBASIC, M
    RAJNINGERMIHOLIC, M
    PRISLINMUSKLIC, M
    DOBROVSAKSOUREK, V
    SMERDEL, S
    STAINER, DW
    [J]. JOURNAL OF BIOLOGICAL STANDARDIZATION, 1989, 17 (01): : 85 - 90
  • [3] BUYNAK EB, 1966, P SOC EXP BIOL MED, V123, P768, DOI 10.3181/00379727-123-31599
  • [4] CIZMAN M, 1989, PEDIATR INFECT DIS J, V8, P302
  • [5] ENDERS JF, 1946, J IMMUNOL, V54, P283
  • [6] PLAQUE FORMATION BY MUMPS VIRUS AND INHIBITION BY ANTISERUM
    FLANAGAN, TD
    BARRON, AL
    [J]. APPLIED MICROBIOLOGY, 1970, 19 (02) : 360 - &
  • [7] DIFFERENTIATION OF VACCINE AND WILD MUMPS VIRUSES USING THE POLYMERASE CHAIN-REACTION AND DIDEOXYNUCLEOTIDE SEQUENCING
    FORSEY, T
    MAWN, JA
    YATES, PJ
    BENTLEY, ML
    MINOR, PD
    [J]. JOURNAL OF GENERAL VIROLOGY, 1990, 71 : 987 - 990
  • [8] FURESZ J, 1987, CAN DIS WEEKLY REP, V13, P156
  • [9] Gluck R, 1986, Dev Biol Stand, V65, P29
  • [10] HAYASHI K, 1976, JPN J EXP MED, V46, P197