CYTOLYTIC ACTIVITY OF HUMAN NATURAL-KILLER-CELL SUBPOPULATIONS ISOLATED BY 4-COLOR IMMUNOFLUORESCENCE FLOW CYTOMETRIC CELL SORTING

被引：46

作者：

SROUR, EF ^{[1
]}

LEEMHUIS, T ^{[1
]}

JENSKI, L ^{[1
]}

REDMOND, R ^{[1
]}

JANSEN, J ^{[1
]}

机构：

[1] PURDUE UNIV,SCH SCI,DEPT BIOL,INDIANAPOLIS,IN 46205

来源：

CYTOMETRY | 1990年 / 11卷 / 03期

关键词：

Large granular lymphocytes; Multiparameter flow cytometric cell sorting; NK subpopulations;

D O I：

10.1002/cyto.990110316

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The natural killer activity and phenotypic properties of six different subpopulations of normal human peripheral blood lymphocytes obtained by four‐color immunofluorescence cell sorting were examined. Phycoerythrin‐conjugated CD16 and CD56 were used simultaneously to identify (CD16+CD56+) NK cells. The cells most effective in mediating NK cytolysis against K562 target cells were CD3−(16+56+)57‐8+. Although most of the K562 killing was found in the CD3−(16+56+) groups of cells, a substantial degree of NK activity was detected in the CD3+(16+56+) subpopulations of some individuals. The level of expression of CD57 and CD8 was significantly higher on CD3+(16+56+) than on CD3−(16+56+) cells. Copyright © 1990 Wiley‐Liss, Inc.

引用

页码：442 / 446

页数：5

共 18 条

[1]

ABO T, 1982, J IMMUNOL, V129, P1758

[2]

ABO T, 1981, J IMMUNOL, V127, P1024

[3] MECHANISM OF K562-INDUCED HUMAN NATURAL-KILLER CELL INACTIVATION USING HIGHLY ENRICHED EFFECTOR-CELLS ISOLATED VIA A NEW SINGLE-STEP SHEEP ERYTHROCYTE ROSETTE ASSAY [J].