DIFFERENCES IN REGULATION BETWEEN NUCLEAR AND CYTOPLASMIC CA2+ IN CULTURED SMOOTH-MUSCLE CELLS

被引:65
作者
HIMPENS, B
DESMEDT, H
DROOGMANS, G
CASTEELS, R
机构
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1992年 / 263卷 / 01期
关键词
NUCLEUS; FREE CALCIUM CONCENTRATION;
D O I
10.1152/ajpcell.1992.263.1.C95
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
The free Ca2+ concentrations in the nucleus ([Ca2+]n) and cytoplasm ([Ca2+]c) of cultured smooth muscle cells were estimated using the fluorescent dye indo-1 and the ACAS 570 confocal laser microscope. In resting DDT1MF2 smooth muscle cells [Ca2+]n was found to be lower than [Ca2+]c. Both values increased transiently in response to histamine (100-mu-M), but during this stimulation [Ca2+]n exceeded [Ca2+]c. Maximal increase of [Ca2+]n was observed in the center of the nucleus, and a maximal increase of [Ca2+]c was observed in the immediate vicinity of the plasma membrane. A similar response was obtained with other agonists, such as carbachol or ATP. Comparable results with ATP were obtained in cultured aorta cells. The differential rise of [Ca2+]n over [Ca2+]c in DDT1MF2 cells did not occur during either spontaneous release of Ca2+ or Ca2+ release induced by caffeine (7.5 mM). The differential rise during histamine stimulation was abolished by the presence of the intercalating substance ethidium bromide. Thapsigargin, a presumed specific inhibitor of the endoplasmic reticulum Ca2+-Mg2+-adenosine-triphosphatase, abolished the Ca2+ gradient between nucleus and cytosol at rest. During subsequent histamine stimulation the Ca2+ increase was largely blocked in both compartments and attained similar levels. We propose that the lower value of (Ca2+]n at rest is dependent on an active Ca2+ extrusion system. The differential rise of [Ca2+]n over [Ca2+]c during agonist stimulation can be explained by an influx of Ca2+ from perinuclear stores and/or by a release of intranuclear Ca2+ possibly mediated by a process dependent on the inositol lipid metabolism.
引用
收藏
页码:C95 / C105
页数:11
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