ANTIBODIES AGAINST THE AMINO-TERMINAL PORTION OF PROENKEPHALIN INHIBIT DNA-SYNTHESIS IN HUMAN PERIPHERAL MONONUCLEAR-CELLS

被引：10

作者：

PADROS, MR ^{[1
]}

SARAVIA, F ^{[1
]}

VINDROLA, O ^{[1
]}

机构：

[1] INST BIOL & MED EXPTL,BUENOS AIRES,DF,ARGENTINA

来源：

JOURNAL OF NEUROIMMUNOLOGY | 1995年 / 61卷 / 01期

关键词：

PROENKEPHALIN; SYNENKEPHALIN; LYMPHOCYTES; PROLIFERATION;

D O I：

10.1016/0165-5728(95)00077-F

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Pro-enkephalin (PENK) mRNA and PENK-derived peptides have been reported in lymphocytes, monocytes, and macrophages. Met-enkephalin (ME) and/or synenkephalin (SYN)-containing peptides are produced and released by human peripheral blood lymphocytes (HPBL) activated with phytohemagglutinin (PHA). Furthermore, SYN (PENK 1-70) was cleaved to low-molecular-mass peptides in HPBL. In this work we studied the effect of a mouse monoclonal antibody (mAb) and a rabbit antiserum (pAb) against the C-terminal portion of SYN on DNA synthesis in PHA-activated HPBL. [H-3]Thymidine incorporation into HPBL incubated with 0.1 mu g/ml of PHA was tested in the presence of different concentrations of mAb immunoglobulin (Ig)G or different dilutions of pAb.mAb induced a concentration-dependent decrease of [H-3]thymidine incorporation into HPBL: 7%, 19%, 28%, and 35% of inhibition was observed with 0.1, 1, 1.5, and 2 mu g IgG, respectively, reaching values of 65% with 10 mu g IgG. Similarly, pAb dilutions of 1/500, 1/1000, 1/2000 and 1/4000 inhibited DNA synthesis by 63%, 61%, 43%, and 30%, respectively. The Inhibitory effect of mAb and pAb was specific since it was not produced by non-immune mouse IgG or several non-immune rabbit sera and was completely reversed by 1 mu M of the synthetic peptide [Tyr(63)](syn 63-70) synenkephalin. These results suggest that low-molecular-mass SYN-derived peptides released by PHA-activated HPBL may participate in the proliferative response of these cells. This is further evidence that the non-opioid portion of PENK-that is, SYN-derived peptides-may be involved in tissue development.

引用

页码：79 / 83

页数：5

共 26 条

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