MATURATION OF HANTAAN VIRUS GLYCOPROTEIN-G1 AND GLYCOPROTEIN-G2

被引:43
作者
ANTIC, D [1 ]
WRIGHT, KE [1 ]
KANG, CY [1 ]
机构
[1] UNIV OTTAWA,FAC MED,DEPT MICROBIOL & IMMUNOL,OTTAWA K1H 8M5,ONTARIO,CANADA
基金
加拿大自然科学与工程研究理事会;
关键词
D O I
10.1016/0042-6822(92)90709-X
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Hantaan virus-infected Vero E6 cell lysates were used for immunoprecipitation with monoclonal antibodies against glycoprotein G1 (MAbG1) or G2 (MAbG2). When cell lysates were prepared with buffer containing nonionic detergent, both G1 and G2 glycoproteins were precipitated with either MAbG1 or MAbG2. In contrast, when cell lysates were prepared with a buffer containing ionic detergents MAbG1 precipitated only glycoprotein G1 and MAbG2 precipitated only glycoprotein G2. Heterodimers and possibly higher oligomeric forms of the glycoproteins were detected on nonreducing SDS-polyacrylamide gels only after chemical cross-linking and immunoprecipitation with either MAbG1 or MAbG2. In order to determine the sites of Hantaan virus glycoproteins maturation and the G1-G2 complex formation, infected cells were treated with inhibitors that prevent specific steps of oligosaccharide processing. Furthermore, glycoproteins G1 and G2 immunoprecipitated from infected cell lysates or from isolated virus particles were tested for sensitivity to endoglycosidase H, endoglycosidase F, and endoglycosidase D. The results of these experiments show that maturation of both G1 and G2 takes place in the endoplasmic reticulum (ER). Furthermore, G1-G2 complex formation occurs in the ER as well, since the two glycoproteins co-precipitated with either MAbG1 or MAbG2 from infected cell lysates treated with brefeldin A and prepared with buffer containing nonionic detergent. © 1992.
引用
收藏
页码:324 / 328
页数:5
相关论文
共 19 条
[1]   MOLECULAR CHARACTERIZATION OF THE M-GENOMIC SEGMENT OF THE SEOUL-80-39 VIRUS - NUCLEOTIDE AND AMINO-ACID-SEQUENCE COMPARISONS WITH OTHER HANTAVIRUSES REVEAL THE EVOLUTIONARY PATHWAY [J].
ANTIC, D ;
LIM, BU ;
KANG, CY .
VIRUS RESEARCH, 1991, 19 (01) :47-58
[2]   NUCLEOTIDE-SEQUENCE AND CODING CAPACITY OF THE LARGE (L) GENOMIC RNA SEGMENT OF SEOUL-80-39 VIRUS, A MEMBER OF THE HANTAVIRUS GENUS [J].
ANTIC, D ;
LIM, BU ;
KANG, CY .
VIRUS RESEARCH, 1991, 19 (01) :59-66
[3]   CHARACTERIZATION OF HANTAAN VIRUS ENVELOPE GLYCOPROTEIN ANTIGENIC DETERMINANTS DEFINED BY MONOCLONAL-ANTIBODIES [J].
ARIKAWA, J ;
SCHMALJOHN, AL ;
DALRYMPLE, JM ;
SCHMALJOHN, CS .
JOURNAL OF GENERAL VIROLOGY, 1989, 70 :615-624
[4]   CODING PROPERTIES OF THE S-GENOME AND THE M-GENOME SEGMENTS OF SAPPORO RAT VIRUS - COMPARISON TO OTHER CAUSATIVE AGENTS OF HEMORRHAGIC-FEVER WITH RENAL SYNDROME [J].
ARIKAWA, J ;
LAPENOTIERE, HF ;
IACONOCONNORS, L ;
WANG, M ;
SCHMALJOHN, CS .
VIROLOGY, 1990, 176 (01) :114-125
[5]   BREFELDIN-A ARRESTS THE MATURATION AND EGRESS OF HERPES-SIMPLEX VIRUS-PARTICLES DURING INFECTION [J].
CHEUNG, P ;
BANFIELD, BW ;
TUFARO, F .
JOURNAL OF VIROLOGY, 1991, 65 (04) :1893-1904
[6]  
FARQUHAR MG, 1985, ANNU REV CELL BIOL, V1, P447, DOI 10.1146/annurev.cb.01.110185.002311
[7]   DETERMINATION OF THE CODING CAPACITY OF THE M-GENOME SEGMENT OF NEPHROPATHIA EPIDEMICA VIRUS-STRAIN HALLNAS B1 BY MOLECULAR-CLONING AND NUCLEOTIDE-SEQUENCE ANALYSIS [J].
GIEBEL, LB ;
STOHWASSER, R ;
ZOLLER, L ;
BAUTZ, EKF ;
DARAI, G .
VIROLOGY, 1989, 172 (02) :498-505
[8]   PROTEIN OLIGOMERIZATION IN THE ENDOPLASMIC-RETICULUM [J].
HURTLEY, SM ;
HELENIUS, A .
ANNUAL REVIEW OF CELL BIOLOGY, 1989, 5 :277-307
[9]  
ORCI L, 1991, CELL, V64, P1183
[10]   NUCLEOTIDE-SEQUENCE ANALYSIS OF THE S-GENOMIC SEGMENT OF PROSPECT HILL VIRUS - COMPARISON WITH THE PROTOTYPE HANTAVIRUS [J].
PARRINGTON, MA ;
KANG, CY .
VIROLOGY, 1990, 175 (01) :167-175