THE PRODUCTION OF FUNCTIONAL SEX-REVERSED MALE RAINBOW-TROUT WITH 17-ALPHA-METHYLTESTOSTERONE AND 11-BETA-HYDROXYANDROSTENEDIONE

被引：74

作者：

FEIST, G ^{[1
]}

YEOH, CG ^{[1
]}

FITZPATRICK, MS ^{[1
]}

SCHRECK, CB ^{[1
]}

机构：

[1] OREGON STATE UNIV,US FISH & WILDLIFE SERV,OREGON COOPERAT FISHERY RES UNIT,CORVALLIS,OR 97331

来源：

AQUACULTURE | 1995年 / 131卷 / 1-2期

关键词：

SEX REVERSAL; ONCORHYNCHUS MYKISS; ANDROGENS;

D O I：

10.1016/0044-8486(94)00336-M

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

The sex of gynogenetic rainbow trout was reversed to produce XX males by using two steroids, 17 alpha-methyltestosterone (MT) and 11 beta-hydroxyandrostenedione (OHA). Fish were exposed to either single or multiple doses of steroids during various times around the period of hatching to determine the labile period for effective sex reversal, Steroids were administered either by immersion (400 mu g/1 for 2 h) or a combination of immersion plus feeding (3 mg/kg diet for 60 days) to determine if males with intact sperm ducts could be produced, Immersion in MT resulted in varying degrees of masculinization while immersion plus feeding produced nearly 100% male populations. The most effective period for steroid immersion was 1 week after the time when one-half of the fish had hatched. Multiple immersions in MT failed to increase masculinizing effects, Immersion in OHA caused only low rates of masculinization, while immersion plus feeding resulted in 70% male populations. Males produced through both immersion and feeding of MT generally did not develop sperm ducts; whereas animals treated by immersion alone in MT, or those produced with OHA, tended to be functional, Although final yields for sex-reversed males following gynogenesis were low, 1.5 and 1.6% for the two groups respectively, cryopreserved semen from these males can be used to produce all-female offspring for years, Cryopreserved semen from functional males in this study produced 100% female populations.

引用

页码：145 / 152

页数：8

共 16 条

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