IDENTIFICATION OF M3, M4 AND M5 SUBTYPES OF MUSCARINIC RECEPTOR MESSENGER-RNA IN HUMAN BLOOD MONONUCLEAR-CELLS

被引：41

作者：

COSTA, P ^{[1
]}

AUGER, CB ^{[1
]}

TRAVER, DJ ^{[1
]}

COSTA, LG ^{[1
]}

机构：

[1] FDN CLIN LAVORO,TOXICOL UNIT,PAVIA,ITALY

来源：

JOURNAL OF NEUROIMMUNOLOGY | 1995年 / 60卷 / 1-2期

关键词：

MACHR SUBTYPES; HUMAN BLOOD MONONUCLEAR CELLS; MESSENGER-RNA;

D O I：

10.1016/0165-5728(95)00051-3

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In this study we made use of the Reverse transcription-polymerase chain reaction to analyze the expression of mRNA for the five subtypes of muscarinic acetylcholine receptors in human blood mononuclear cells. mRNA for m3, m4 and m5 subtypes was detected, while mRNA for mi and m2 muscarinic receptors was not found. Similar results were obtained for three different healthy human subjects studied. Interestingly, the m5 subtype was expressed at higher levels in blood mononuclear cells than in cerebral cortex. To our knowledge this is the first time that m5 muscarinic receptor mRNA has been found outside of the central nervous system.

引用

页码：45 / 51

页数：7

共 32 条

[1] A MUSCARINIC RECEPTOR TYPE IN HUMAN-LYMPHOCYTES - A COMPARISON OF H-3 QNB BINDING TO INTACT LYMPHOCYTES AND LYSED LYMPHOCYTE MEMBRANES [J].