RANDOM AMPLIFICATION OF POLYMORPHIC BACTERIAL-DNA - EVALUATION OF 11 OLIGONUCLEOTIDES AND APPLICATION TO FOOD CONTAMINATED WITH LISTERIA-MONOCYTOGENES

被引：25

作者：

NIEDERHAUSER, C ^{[1
]}

HOFELEIN, C ^{[1
]}

ALLMANN, M ^{[1
]}

BURKHALTER, P ^{[1
]}

LUTHY, J ^{[1
]}

CANDRIAN, U ^{[1
]}

机构：

[1] UNIV BERN,INST BIOCHEM,FOOD CHEM LAB,CH-3012 BERN,SWITZERLAND

来源：

JOURNAL OF APPLIED BACTERIOLOGY | 1994年 / 77卷 / 05期

关键词：

D O I：

10.1111/j.1365-2672.1994.tb04404.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The polymerase chain reaction was used to obtain randomly-amplified polymorphic DNA (RAPD) profiles from Listeria spp. and enterobacteria. Eleven different oligonucleotides were evaluated. Only one, HR4 (19mer), generated reproducible and specific profiles for Listeria spp., while results for enterobacteria were controversial. A total of 57 different Listeria strains were subjected to the RAPD analysis and 27 different profiles were recognized. RAPD typing allowed strains of the same serotype to be distinguished but the same profile was obtained from different serotypes oft. monocytogenes in three cases and in one case two different serotypes of L. innocua yielded the same profile. RAPD-typing with HR4 allowed L. monocytogenes contamination in several food outlets to be traced back to a food processing plant. In additional experiments, the general utility of this RAPD system in typing Yersinia enterocolitica, verotoxigenic Escherichia coli and Salmonella enteriridis was evaluated.

引用

页码：574 / 582

页数：9

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