LECTINS (HEMAGGLUTININS) IN THE HEMOLYMPH OF GLOSSINA-FUSCIPES-FUSCIPES - ISOLATION, PARTIAL CHARACTERIZATION, SELECTED PHYSICOCHEMICAL PROPERTIES AND CARBOHYDRATE-BINDING SPECIFICITIES

被引:29
作者
INGRAM, GA
MOLYNEUX, DH
机构
[1] Department of Biological Sciences, University of Salford, Salford M5 4WT, Lancashire England
来源
INSECT BIOCHEMISTRY | 1990年 / 20卷 / 01期
关键词
Glossina fuscipes fuscipes; haemagglutinins; haemolymph; isolation; lectins; physico-chemical properties; sugar specificity;
D O I
10.1016/0020-1790(90)90017-O
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Glossina fuscipes fuscipes haemolymph contained agglutinins (lectins), titre range 2-11-2-18, against red blood cells (RBC) of human ABO(H) blood group with highest values detected against "AB" RBC. The use of protease- and neuraminidase-treated RBC in many cases increased titres whilst treatment with galactosidases or glucosidases caused decreased levels. Haemolymph adsorption with "O" RBC reduced titres against "O" and "AB" but to a lesser extent anti-A or -B activity indicating lectin heterogeneity. The carbohydrate-binding specificities for human RBC were directed towards N-acetylated and deoxy derivatives of glucose and/or galactose. In addition the haemagglutinins were reactive against some oligosaccharides, ribose, deoxymannose, deoxygalactose, xylose and xylan with certain of the RBC types. The agglutinins were glycoprotein in nature, thermo-labile, affected by storage, freezing and thawing treatments and exposure to a high dosage of γ-radiation, possessed limited disulphide and hydrogen bonds, and depended upon slightly acid to neutral conditions for optimum agglutination. The haemag-glutinins did not require the presence of divalent cations (Ca2+, Mn2+ or Cu2+ ions) for activity although an elevated concentration of Mg2+ ions resulted in increased endpoint titres. However heavy metal ions (Pb2+ and Fe2+) in the buffer lowered agglutinin levels. The intact lectin molecule had an isoelectric point of 6.2, a relative molecular weight of 710 kDa and comprised approx. 70 kDa subunits. © 1990.
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页码:13 / 27
页数:15
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