REDUCED LEVELS OF TRANSFORMING GROWTH-FACTOR-BETA TYPE-I RECEPTOR IN HUMAN GASTRIC CARCINOMAS

被引：50

作者：

ITO, M ^{[1
]}

YASUI, W ^{[1
]}

NAKAYAMA, H ^{[1
]}

YOKOZAKI, H ^{[1
]}

ITO, H ^{[1
]}

TAHARA, E ^{[1
]}

机构：

[1] HIROSHIMA UNIV,SCH MED,DEPT PATHOL 1,1-2-3 KASUMI,MINAMI KU,HIROSHIMA 734,JAPAN

来源：

JAPANESE JOURNAL OF CANCER RESEARCH | 1992年 / 83卷 / 01期

关键词：

TGF-BETA; TGF-BETA RECEPTOR; TGF-BETA INHIBITORY ELEMENT; GASTRIC CARCINOMA;

D O I：

10.1111/j.1349-7006.1992.tb02356.x

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The expressions of transforming growth factor beta (TGF-beta) and its receptor and TGF-beta inhibitory element (TIE)-binding protein were examined on human gastric carcinomas by Northern blot hybridization, immunohistochemistry, affinity labeling and gel retardation analysis. TGF-beta mRNA was expressed in tumor and normal tissues at various levels. Immunohistochemically, TGF-beta expression was confirmed to be present within tumor cells. Out of the 17 human gastric carcinoma tissues, 14 (82%) showed a reduction in the level of type I receptor (65 kDa) for TGF-beta when compared to corresponding normal mucosas. Interestingly, in seven of the 14 tumors the level of TIE-binding protein in the tumor tissue was lower than that in normal mucosa. Human gastric carcinoma cell line TMK-1, whose growth was inhibited by TGF-beta, had only type I receptor for TGF-beta and showed a high level of TIE-binding protein. Conversely, MKN-1, a TGF-beta-resistant cell line, exhibited an extremely low level of TGF-beta receptor and had no TIE-binding protein. These results overall indicate that although human gastric carcinoma cells produced TGF-beta, they showed a reduction in TGF-beta type I receptor and a low level of TIE-binding protein, resulting in escape from growth inhibition by TGF-beta.

引用

页码：86 / 92

页数：7

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