MEASUREMENT OF EICOSANOIDS IN MENSTRUAL FLUID BY THE COMBINED USE OF HIGH-PRESSURE CHROMATOGRAPHY AND RADIOIMMUNOASSAY

被引：31

作者：

HOFER, G

BIEGLMAYER, C

KOPP, B

JANISCH, H

机构：

[1] UNIV HOSP VIENNA,INST MED & CHEM LAB DIAGNOST,WAHRINGERGURTEL 18-20,A-1090 VIENNA,AUSTRIA

[2] UNIV HOSP VIENNA,DEPT OBSTET & GYNECOL 2,A-1090 VIENNA,AUSTRIA

[3] UNIV VIENNA,INST PHARMACOGNOST,A-1010 VIENNA,AUSTRIA

来源：

PROSTAGLANDINS | 1993年 / 45卷 / 05期

关键词：

D O I：

10.1016/0090-6980(93)90118-Q

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Methods are described for the quantification of various eicosanoids (cyclooxygenase products: 6-KETO, TXB2, PGE2, PGF2alpha, DHK; lipoxygenase products: 5-, 12-, 15-HETE, LTB4, LTC4, LTD4, LTE4) in menstrual blood collected by tampons. Samples were extracted with acidified ethanol. After purification by SEP-PACK C18 columns, the compounds were separated by reversed phase HPLC using a ternary gradient system. The eicosanoid concentrations of the fractionated eluents were measured by radioimmunoassay and corrected for recovery. 12-HETE was the most prominent metabolite of arachidonic acid in menstrual blood (mean: 1174 ng/g blood). With the exception of PGF2alpha and TXB2 (mean: 343 and 212 ng/g blood, respectively) other eicosanoids were detected in remarkable lower concentrations. Supported by grant #P7137 from the Austrian ''Fonds zur Forderung der wissenschaftlichen Forschung''

引用

页码：413 / 426

页数：14

共 16 条

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