MOSQUITO SENSITIVITY TO A SCORPION NEUROTOXIN EXPRESSED USING AN INFECTIOUS SINDBIS-VIRUS VECTOR

The scorpion, Androctonus australis Hector, produces an insect-specific toxin (AaHIT) encoded by the Scoter gene. To assess the toxicity of AaHIT for mosquitoes, we have taken a novel approach to express the Scoter gene in vivo, We have engineered a double subgenomic Sindbis (dsSIN) virus that contains the Scoter gene in the viral genome and intrathoracically inoculated the virus (TE/3'2J/Scotox) into mosquitoes (Aedes aegypti, Ae. triseriatus and Culex pipiens), houseflies (Musca domestica) and ticks (Dermacentor andersoni), Mosquitoes, which normally show no pathologic effects from Sindbis (SIN) virus infections, died 1-5 days after infection with TE/3'2J/Scotox virus. Neither flies nor ticks were killed, The mosquitocidal action of AaHIT in mosquitoes makes AaHIT a potential candidate for inclusion in molecular-based methods of mosquito control. The expression of an arthropod gene in vivo demonstrates the utility of dsSIN expression vectors for future use to examine and potentially disrupt endogenous gene functions in mosquitoes.