METHODS FOR ISOLATION AND CHARACTERIZATION OF CONSTITUENTS OF NATURAL PRODUCTS .8. GAS-LIQUID CHROMATOGRAPHIC RESOLUTION OF ALCOHOL ESTER AMIDE AND THIOESTER DERIVATIVES OF PYRUVIC ACID 2,6-DINITROPHENYLHYDRAZONE

Gas-liquid chromatographic techniques have been employed for the resolution of homologous series of primary, secondary, and tertiary alcohol ester- primary amine amide- and thioester derivatives of pyruvic acid 2,6-dinitrophenylhydrazone. The derivatives can be separated isothermally or by temperature programming on 6.5 or 4-foot J × R or on a 1-foot SE 30 column and can be detected by flame or electron capture detectors. The latter permits the detection of from 10-15 mμg of a derivative which is equivalent to about 1-5 mμg of the parent compound. © 1968.