ISOLATION BY POLYMERASE CHAIN-REACTION OF A CDNA WHOSE PRODUCT PARTIALLY COMPLEMENTS THE ULTRAVIOLET SENSITIVITY OF XERODERMA-PIGMENTOSUM GROUP-C CELLS

被引：20

作者：

TEITZ, T ^{[1
]}

PENNER, M ^{[1
]}

ELI, D ^{[1
]}

STARK, M ^{[1
]}

BAKHANASHVILI, M ^{[1
]}

NAIMAN, T ^{[1
]}

CANAANI, D ^{[1
]}

机构：

[1] TEL AVIV UNIV,GEORGE S WISE FAC LIFE SCI,DEPT BIOCHEM,IL-69978 TEL AVIV,ISRAEL

来源：

GENE | 1990年 / 87卷 / 02期

关键词：

expressible cDNA library; gene transfer; human disease; irradiation; Recombinant DNA;

D O I：

10.1016/0378-1119(90)90316-J

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A xeroderma pigmentosum (XP) cell line from complementation group C has been complemented to attain ultraviolet (UV) resistance and DNA repair proficiency, by transfection with a human expression cDNA library, followed by selection to UV resistance. We now show that the transfected cDNAs can be rescued from cellular DNA of a secondary transformant by its in vitro amplification using expression-vector-specific oligodeoxyribonucleotides as primers in a polymerase chain reaction. The amplified cDNAs were cloned into a mammalian expression vector. Their transfection into XP cells identified a single cDNA which specifically complemented the UV sensitivity of a group C-derived cell line to the same partial UV-resistance levels exhibited by the transformant from which the cDNAs were rescued. © 1990.

引用

页码：295 / 298

页数：4

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