AN IMMUNODOMINANT N-TERMINAL REGION OF VP1 PROTEIN OF POLIOVIRION THAT IS BURIED IN CRYSTAL-STRUCTURE CAN BE EXPOSED IN SOLUTION

被引：62

作者：

ROIVAINEN, M

PIIRAINEN, L

RYSA, T

NARVANEN, A

HOVI, T

机构：

[1] NATL PUBL HLTH INST,MOLEC BIOL PROGRAM,SF-00280 HELSINKI 28,FINLAND

[2] LABSYST RES LABS,HELSINKI,FINLAND

来源：

VIROLOGY | 1993年 / 195卷 / 02期

关键词：

D O I：

10.1006/viro.1993.1427

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

According to previous X-ray crystallographic analyses of poliovirus structure, the amino terminal region of the capsid protein VP1 is inside the capsid shell. We have found that antibodies to synthetic peptides derived from an immunodominant part of this region readily precipitate intact infectious virions, indicating that in solution, the virus can assume a conformation that is partially different from that seen in the crystal structure. We propose that this part of VP1 is alternating between two conformations. © 1993 Academic Press. All rights reserved.

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页码：762 / 765

页数：4

相关论文

共 25 条

[1] THE 3-DIMENSIONAL STRUCTURE OF FOOT-AND-MOUTH-DISEASE VIRUS AT 2.9-A RESOLUTION [J].

ACHARYA, R ;

FRY, E ;

STUART, D ;

FOX, G ;

ROWLANDS, D ;

BROWN, F .

NATURE, 1989, 337 (6209) :709-716

[2] MUTATIONS CONFERRING RESISTANCE TO NEUTRALIZATION WITH MONOCLONAL-ANTIBODIES IN TYPE-1 POLIOVIRUS CAN BE LOCATED OUTSIDE OR INSIDE THE ANTIBODY-BINDING SITE [J].

BLONDEL, B ;

CRAINIC, R ;

FICHOT, O ;

DUFRAISSE, G ;

CANDREA, A ;

DIAMOND, D ;

GIRARD, M ;

HORAUD, F .

JOURNAL OF VIROLOGY, 1986, 57 (01) :81-90

[3] SYNTHETIC PEPTIDES FROM 4 SEPARATE REGIONS OF THE POLIOVIRUS TYPE-1 CAPSID PROTEIN VP1 INDUCE NEUTRALIZING ANTIBODIES [J].

CHOW, M ;

YABROV, R ;

BITTLE, J ;

HOGLE, J ;

BALTIMORE, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (03) :910-914

[4] ANTIGENIC VARIATION AND RESISTANCE TO NEUTRALIZATION IN POLIOVIRUS TYPE-1 [J].

DIAMOND, DC ;

JAMESON, BA ;

BONIN, J ;

KOHARA, M ;

ABE, S ;

ITOH, H ;

KOMATSU, T ;

ARITA, M ;

KUGE, S ;

NOMOTO, A ;

OSTERHAUS, ADME ;

CRAINIC, R ;

WIMMER, E .

SCIENCE, 1985, 229 (4718) :1090-1093

[5] IDENTIFICATION OF A NEW NEUTRALIZATION ANTIGENIC SITE ON POLIOVIRUS COAT PROTEIN-VP2 [J].

EMINI, EA ;

JAMESON, BA ;

WIMMER, E .

JOURNAL OF VIROLOGY, 1984, 52 (02) :719-721

[6] PRIMING FOR AND INDUCTION OF ANTI-POLIOVIRUS NEUTRALIZING ANTIBODIES BY SYNTHETIC PEPTIDES [J].

EMINI, EA ;

JAMESON, BA ;

WIMMER, E .

NATURE, 1983, 304 (5928) :699-703

[7] STRUCTURAL FACTORS THAT CONTROL CONFORMATIONAL TRANSITIONS AND SEROTYPE SPECIFICITY IN TYPE-3 POLIOVIRUS [J].

FILMAN, DJ ;

SYED, R ;

CHOW, M ;

MACADAM, AJ ;

MINOR, PD ;

HOGLE, JM .

EMBO JOURNAL, 1989, 8 (05) :1567-1579

[8] CELL-INDUCED CONFORMATIONAL CHANGE IN POLIOVIRUS - EXTERNALIZATION OF THE AMINO TERMINUS OF VP1 IS RESPONSIBLE FOR LIPOSOME BINDING [J].

FRICKS, CE ;

HOGLE, JM .

JOURNAL OF VIROLOGY, 1990, 64 (05) :1934-1945

[9] USE OF PEPTIDE-SYNTHESIS TO PROBE VIRAL-ANTIGENS FOR EPITOPES TO A RESOLUTION OF A SINGLE AMINO-ACID [J].

GEYSEN, HM ;

MELOEN, RH ;

BARTELING, SJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (13) :3998-4002

[10] 3-DIMENSIONAL STRUCTURE OF POLIOVIRUS AT 2.9 A RESOLUTION [J].

HOGLE, JM ;

CHOW, M ;

FILMAN, DJ .

SCIENCE, 1985, 229 (4720) :1358-1365