PROTEIN KINASE-A MODULATES AN ENDOGENOUS CALCIUM-CHANNEL, BUT NOT THE CALCIUM-ACTIVATED CHLORIDE CHANNEL, IN XENOPUS-OOCYTES

被引：5

作者：

CHEN, Y

POLLOCK, JD

WANG, YH

DEPAOLIROACH, AA

YU, L

机构：

[1] INDIANA UNIV, SCH MED, DEPT MED & MOLEC GENET, 975 W WALNUT ST, INDIANAPOLIS, IN 46202 USA

[2] INDIANA UNIV, SCH MED, DEPT PHYSIOL & BIOPHYS, INDIANAPOLIS, IN 46202 USA

[3] INDIANA UNIV, SCH MED, DEPT BIOCHEM & MOLEC BIOL, INDIANAPOLIS, IN 46202 USA

来源：

FEBS LETTERS | 1993年 / 336卷 / 02期

关键词：

PROTEIN KINASE-A; PHOSPHATASE; CALCIUM CHANNEL; XENOPUS-OOCYTE; CALCIUM IONOPHORE;

D O I：

10.1016/0014-5793(93)80800-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In Xenopus oocytes, Ca2+ influx through an endogenous voltage-gated Ca2+ channel activates a transient outward Cl- current (I(Cl(Ca)), which is potentiated by cAMP increase. The site of cAMP effect appears to be the Ca2+ channel instead of the Ca2+-activated Cl- channel, because cAMP potentiates the Ba2+ current through the Ca2+ channel in a similar way to the I(Cl(Ca)), and cAMP does not potentiate the Ca2+-dependent Cl- current in cells treated with Ca2+ ionophore. Using the catalytic subunit of protein kinase A (PKA) and PKA inhibitors, it was shown that PKA is both necessary and sufficient for the cAMP effect on I(Cl(Ca)). Furthermore, the cAMP/PKA-mediated potentiation of I(Cl(Ca)), was inhibited by both type 1 and type 2A protein phosphatases.

引用

页码：191 / 196

页数：6

共 26 条

[1] A TRANSIENT CALCIUM-DEPENDENT CHLORIDE CURRENT IN THE IMMATURE XENOPUS OOCYTE [J].