APPLICATION OF CAPILLARY ELECTROPHORESIS, HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, ONLINE ELECTROSPRAY MASS-SPECTROMETRY AND MATRIX-ASSISTED LASER-DESORPTION IONIZATION-TIME OF FLIGHT MASS-SPECTROMETRY TO THE CHARACTERIZATION OF SINGLE-CHAIN PLASMINOGEN-ACTIVATOR

被引：25

作者：

APFFEL, A ^{[1
]}

CHAKEL, J ^{[1
]}

UDIAVAR, S ^{[1
]}

HANCOCK, WS ^{[1
]}

SOUDERS, C ^{[1
]}

PUNGOR, E ^{[1
]}

机构：

[1] BERLEX BIOSCI,BRISBANE,CA 94005

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 717卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(95)00603-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The analysis of recombinant Desmodus salivary plasminogen activator (DSPA alpha 1), a heterogeneous glycoprotein, is demonstrated through the use of high-performance liquid chromatography (HPLC), high-performance capillary electrophoresis (HPCE), liquid chromatography-electrospray mass spectrometry (LC-ES-MS), and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS). The protein is analyzed at three specific levels of detail: the intact protein, proteolytic digests of the protein, and fractions from the proteolytic digest. A method for ''on-column'' collection of HPLC fractions for subsequent transfer and analysis by HPCE and MALDI-TOF-MS is shown.

引用

页码：41 / 60

页数：20

共 22 条

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