ISOLATION, SEQUENCE, AND BIOACTIVITY OF FMRFAMIDE-RELATED PEPTIDES FROM THE LOCUST VENTRAL NERVE CORD

被引：53

作者：

LANGE, AB ^{[1
]}

PEEFF, NM ^{[1
]}

ORCHARD, I ^{[1
]}

机构：

[1] UNIV TORONTO,DEPT ZOOL,TORONTO M5S 1A1,ON,CANADA

来源：

PEPTIDES | 1994年 / 15卷 / 06期

基金：

加拿大自然科学与工程研究理事会;

关键词：

FMRFAMIDE-RELATED PEPTIDES; HPLC; RIA; CONTRACTION; OVIDUCT; LOCUST;

D O I：

10.1016/0196-9781(94)90075-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The ventral nerve cord of the locust, Locusta migratoria, was examined for the presence of FMRFamide-related peptides (FaRPs). RP-HPLC coupled to an RIA specific for extended -RFamides revealed the presence of several FaRPs eluting at different percentages of acetonitrile. The sequences of five of these peptides were determined. Two sequences are identical to the two peptides previously sequenced from brain and retrocerebral complex of Locusta, These two peptides (PDVDHVFLRFamide and ADVGHVFLRFamide) were inhibitory when tested on locust oviduct contractions. The other peptides are novel with sequences of GQERNFLRFamide, AXXRNFIRFamide, and AFIRFamide. The synthesized peptides were stimulatory when tested on locust oviduct contractions, increasing the frequency and amplitude of spontaneous contractions and resulting in a basal contraction.

引用

页码：1089 / 1094

页数：6

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