SENSITIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS FOR TOXICOLOGICAL STUDIES WITH CARBARYL

Carbaryl and its degradation products could be detected in water and in serum at levels as low as 0.5 ng/mL (or 5% full scale responses at 0.005 aufs) by HPLC analysis using a 3-mu-m C18 Ultrasphere column on a Beckman HPLC System (Beckman, Inc., San Ramon, CA). Preparation included a single extraction with methanol after application of samples to 1.0 mL of C18 solid-phase extraction columns. Advantages of this method include high sensitivity, analysis time of 10 min after a single extraction, and high recovery. Sensitivity was maintained whether the carbaryl or its degradation products or metabolites were provided in water or in serum. This HPLC method would be useful for toxicological studies in which detection of low concentrations of carbaryl were needed.