HUMAN DIHYDROLIPOAMIDE SUCCINYLTRANSFERASE - CDNA CLONING AND LOCALIZATION ON CHROMOSOME 14Q24.2-Q24.3

被引：26

作者：

NAKANO, K

MATUDA, S

SAKAMOTO, T

TAKASE, C

NAKAGAWA, S

OHTA, S

ARIYAMA, T

INAZAWA, J

ABE, T

MIYATA, T

机构：

[1] KANOYA NATL INST FITNESS & SPORTS,DEPT BIOL,KANOYA,KAGOSHIMA 89123,JAPAN

[2] KAGOSHIMA WOMENS JR COLL,DEPT BIOCHEM,KAGOSHIMA 890,JAPAN

[3] KAGOSHIMA UNIV,SCH MED,DEPT ANAT,KAGOSHIMA 890,JAPAN

[4] JICHI MED SCH,DEPT BIOCHEM,MINAMI KAWACHI,TOCHIGI 32904,JAPAN

[5] KYOTO PREFECTURAL UNIV MED,DEPT HYG,KYOTO 602,JAPAN

[6] KYOTO UNIV,FAC SCI,DEPT BIOL,KYOTO 606,JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1216卷 / 03期

关键词：

DIHYDROLIPOAMIDE SUCCINYLTRANSFERASE; ALPHA-KETOGLUTARATE DEHYDROGENASE COMPLEX; CHROMOSOMAL LOCALIZATION; (HUMAN);

D O I：

10.1016/0167-4781(93)90002-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We isolated cDNA for dihydrolipoamide succinyltransferase from a human fibroblast cDNA library in lambda gt11. The cDNA revealed that the human dihydrolipoamide succinyltransferase lacked a sequence motif of an E3 and/or E1 binding site. This suggests that the human dihydrolipoamide succinyltransferase possesses a unique structure consisting of two domains in contrast with the dihydrolipoamide acyltransferases of other alpha-keto acid dehydrogenase complexes. In addition, we found that the human dihydrolipoamide succinyltransferase gene is located on chromosome 14 at q24.2-924.3 and that a sequence related to the dihydrolipoamide succinyltansferase gene is located on chromosome 1 at p31. Interestingly, the gene for the dihydrolipoamide acyltransferase of the branched chain alpha-keto acid dehydrogenase complex is also located on chromosome 1p31 (Zneimer et al. (1991) Genomics 10, 740-747).

引用

页码：360 / 368

页数：9

共 34 条

[1] COMPARISON OF THE AMINO-ACID SEQUENCES OF THE TRANSACYLASE COMPONENTS OF BRANCHED-CHAIN OXOACID DEHYDROGENASE OF PSEUDOMONAS-PUTIDA, AND THE PYRUVATE AND 2-OXOGLUTARATE DEHYDROGENASES OF ESCHERICHIA-COLI [J].