TISSUE-TO-SOLVENT RATIO AND OTHER FACTORS AFFECTING DETERMINATION OF SOLUBLE POLYPHENOLS IN TROPICAL LEAVES

被引：26

作者：

CONSTANTINIDES, M

FOWNES, JH

机构：

[1] Department of Agronomy and Soil Science, University of Hawaii, Honolulu, HI 96822

来源：

COMMUNICATIONS IN SOIL SCIENCE AND PLANT ANALYSIS | 1994年 / 25卷 / 19-20期

关键词：

D O I：

10.1080/00103629409369260

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Soluble polyphenol concentrations of tropical leaves are believed to affect nitrogen (N) mineralization potential during decomposition. However, polyphenol concentrations reported for the same species have varied several-fold among studies. Testing potential explanations for these discrepancies, we found high tissue-to-solvent ratios, as specified in one of two popular extraction procedures, led to reduced estimates. Use of aqueous methanol rather than water increased recovered polyphenols somewhat, but was not adequate to explain differences among studies. Elevated drying temperatures decreased recovery of soluble polyphenols. Folin-Ciocalteu reagent produced less precipitate and was more reproducible than Folin-Denis reagent.

引用

页码：3221 / 3227

页数：7

共 22 条

[1] QUALITY ASSESSMENT OF TROPICAL BROWSE LEGUMES - TANNIN CONTENT AND PROTEIN-DEGRADATION [J].