DEVELOPMENTAL IMMUNOREACTIVITY FOR GABA AND GAD IN THE AVIAN RETINA - POSSIBLE ALTERNATIVE PATHWAY FOR GABA SYNTHESIS

Although the distribution of GABAergic neurons in chick retina has been previously described by several investigators, the early appearance of these neurons has not been reported. In the present study immunohistochemical methods were used to localize GABAergic neurons with antisera to both GABA and its synthesizing enzyme, glutamate decarboxylase (GAD), in embryonic chick retina at several stages of development and beyond hatching. GABA-positive neuroblast-like cells were clearly detected in retinas as early as embryonic day 6. In contrast, GAD-containing cells were not observed in retinas until embryonic day 10. These findings indicated that immunocytochemically detectable amounts of GAD were not present in young GABAergic cells. Our data on the developmental appearance of GABA and GAD immunoreactivities are consistent with previous biochemical data for the development of GABA concentration and GAD activity in the chick retina. Together, these data suggest that retina cell from the early stages of development may synthesize GABA from an alternative pathway in which the most likely precursor is putrescine. © 1990.