COMPARATIVE AUTO-CONTROLLED STUDY BETWEEN SWIM-UP AND PERCOLL PREPARATION OF FRESH SEMEN SAMPLES FOR INVITRO FERTILIZATION

被引：45

作者：

ENGLERT, Y

VANDENBERGH, M

RODESCH, C

BERTRAND, E

BIRAMANE, J

LEGREVE, A

机构：

[1] Fertility Unit, Department of Obstetrics/Gynaecology, Erasmus Hospital-ULB, 1070 Brussels, Route de Lennik

来源：

HUMAN REPRODUCTION | 1992年 / 7卷 / 03期

关键词：

IVF; SPERM PREPARATION; SWIM-UP; PERCOLL GRADIENTS;

D O I：

10.1093/oxfordjournals.humrep.a137657

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

This study compared swim-up and Percoll preparation of fresh semen samples for in-vitro fertilization. Sixty trials of in-vitro fertilization (IVF), 38 with normal semen and 22 with abnormal semen, comprising 734 oocytes were included in the study. Each semen sample was prepared by both a swim-up technique and a simplified discontinuous (50%, 70%, 90%) Percoll gradient. The oocytes for each trial were distributed at random between the two sperm preparations and incubated with the same number of motile spermatozoa. Percoll gradient preparation produced a significantly higher final concentration of spermatozoa than swim-up preparation (mean +/- SEM: 6.6 +/- 1.5 x 10(6)/ml versus 1.9 +/- 0.2 x 10(6)/ml ; P < 0.01) but a significantly lower sperm motility (69 +/- 2% versus 94 +/- 1%; P < 0.001) and a lower number of normal forms (55 +/- 2% versus 64 +/- 2%; P < 0.01). The ability of the Percoll gradient method to extract motile spermatozoa was higher than that of the swim-up technique (20 +/- 15.6% versus 0.8 +/- 13.6%). Nevertheless, the rates of fertilization (61%), fertilization failure (18%) and polyspermia (9%), embryo quality evaluated by mean embryo scores (3.8 +/- 0.3) and the mean number of spare embryos frozen per trial (1.4 +/- 0.3) were strictly identical in both groups. The 24 pregnancies (including three from frozen - thawed embryos) obtained in these 60 trials (40% per oocyte retrieval) could not be separated according to the sperm preparation method, as embryos from both groups were replaced together. This study provides evidence for the importance of intrinsic sperm fertility in the IVF outcome, making it apparently more important than the preparation technique employed.

引用

页码：399 / 402

页数：4

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