COMPARATIVE DETERMINATION OF SALINOMYCIN BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, MICROBIOLOGICAL AND COLORIMETRIC METHODS IN TESTING PRODUCTION PROCESSES AND ANIMAL FEED PREPARATIONS

Salinomycin and other polyether antibiotics usually do not have a strong chromophore, nor electrochemical or fluorescence activity. Consequently, common liquid chromatography detectors cannot be used for the direct determination of these substances and LC methods that utilize a specific post-column reaction with vanillin have been developed to overcome this limitation. In this report, some modifications of previously published LC methods were made to optimize the analysis of salinomycin. Comparison with standard microbiological and colorimetric methods has been carried out. The LC method was found to provide the best way of precise, and accurate determination in low levels of salinomycin down to the concentration of 1 mg kg-1.