EFFECTS OF THE 3 PROTEASES FROM GUT JUICE OF THE SILKWORM, BOMBYX-MORI, ON THE 2 MORPHOLOGICALLY DIFFERENT INCLUSIONS OF DELTA-ENDOTOXIN PRODUCED BY BACILLUS-THURINGIENSIS-KURSTAKI HD-1 STRAIN

被引:16
作者
TOJO, A [1 ]
SAMASANTI, W [1 ]
YOSHIDA, N [1 ]
AIZAWA, K [1 ]
机构
[1] KYUSHU UNIV, FAC SCI, DEPT CHEM, FUKUOKA 812, JAPAN
来源
AGRICULTURAL AND BIOLOGICAL CHEMISTRY | 1986年 / 50卷 / 03期
关键词
D O I
10.1080/00021369.1986.10867436
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Three proteases designated P-I, P-II, and P-III were separated from larval gut juice of the silkworm, Bombyx mori, and the effects of these proteases on the two morphologically different inclusions produced by Bacillus thuringiensis kurstaki HD-1 strain were investigated. Bipyramidal inclusions were solubilized by each protease at pH 10.2, and proteins with a molecular weight of about 60,000 were produced, while cuboidal inclusions were resistant to the proteases. Protease P-III appeared to be most important in the dissolution of the bipyramidal inclusions. Based on the affinity to various synthesized substrates, P-III was considered to be a chymotrypsin-like protease. After dissolution of inclusions by P-III, dissolved and undissolved fractions were separated, and the insecticidal activity of each fraction was tested. Results indicate that bipyramidal inclusions are preferentially toxic to the silkworm and the cuboidal ones carry the mosquitocidal activity.
引用
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页码:575 / 580
页数:6
相关论文
共 20 条
[1]  
HALL IM, 1977, MOSQ NEWS, V37, P246
[2]   POSSIBLE LOCATION OF THE MOSQUITOCIDAL PROTEIN IN THE CRYSTAL PREPARATION OF BACILLUS-THURINGIENSIS SUBSP KURSTAKI [J].
IIZUKA, T ;
YAMAMOTO, T .
FEMS MICROBIOLOGY LETTERS, 1983, 19 (2-3) :187-192
[3]  
ITZAKI RF, 1964, ANAL BIOCHEM, V9, P401
[4]  
KASSELL B, 1970, METHOD ENZYMOL, V19, P853
[5]   CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].
LAEMMLI, UK .
NATURE, 1970, 227 (5259) :680-+
[6]  
LECADET MM, 1966, B SOC CHIM BIOL, V48, P661
[7]  
LECADET MM, 1966, B SOC CHIM BIOL, V48, P631
[8]   SIMPLIFIED METHOD FOR CYANOGEN-BROMIDE ACTIVATION OF AGAROSE FOR AFFINITY CHROMATOGRAPHY [J].
MARCH, SC ;
PARIKH, I ;
CUATRECASAS, P .
ANALYTICAL BIOCHEMISTRY, 1974, 60 (01) :149-152
[9]  
NAKAJIMA K, 1979, J BIOL CHEM, V254, P4027
[10]  
SAMASANTI W, 1983, ANN REP ICME OSAKA U, V6, P233