IDENTIFICATION AND ELIMINATION OF DNA-SEQUENCES IN TAQ DNA-POLYMERASE

被引：81

作者：

HUGHES, MS

BECK, LA

SKUCE, RA

机构：

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1994年 / 32卷 / 08期

关键词：

D O I：

10.1128/JCM.32.8.2007-2008.1994

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

This study confirms that different preparations of Tag DNA polymerase are contaminated with eubacterial DNA. The contaminants appeared to represent more than one strain or species but were not identified as Thermus aquaticus or Escherichia coli. Differences in microcentrifuge tube composition appeared to affect elimination of the contaminants.

引用

页码：2007 / 2008

页数：2

共 5 条

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[2] IDENTIFICATION OF MYCOBACTERIA FROM ANIMALS BY RESTRICTION ENZYME ANALYSIS AND DIRECT DNA-CYCLE SEQUENCING OF POLYMERASE CHAIN REACTION-AMPLIFIED 16S RIBOSOMAL-RNA GENE-SEQUENCES
HUGHES, MS
SKUCE, RA
BECK, LA
NEILL, SD
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (12) : 3216 - 3222
[3] ELIMINATION OF CONTAMINATING DNA WITHIN POLYMERASE CHAIN-REACTION REAGENTS - IMPLICATIONS FOR A GENERAL-APPROACH TO DETECTION OF UNCULTURED PATHOGENS
MEIER, A
PERSING, DH
FINKEN, M
BOTTGER, EC
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (03) : 646 - 652
[4] TAQ POLYMERASE CONTAINS BACTERIAL-DNA OF UNKNOWN ORIGIN
RAND, KH
HOUCK, H
[J]. MOLECULAR AND CELLULAR PROBES, 1990, 4 (06) : 445 - 450
[5] SCHMIDT TM, 1991, BIOTECHNIQUES, V11, P176

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