2'-(3-ALPHA-BENZYLOXY-24-NORCHOLAN-23-YL)-2',4',4'-TRIMETHYL-4',5'-DIHYDROOXAZOLINE-N-OXYL AS A POTENTIAL SPIN-PROBE FOR MODEL MEMBRANES

被引：8

作者：

BANERJEE, S

TRIVEDI, GK

SRIVASTAVA, S

PHADKE, RS

机构：

[1] INDIAN INST TECHNOL, DEPT CHEM, BOMBAY 400076, INDIA

[2] TATA INST FUNDAMENTAL RES, CHEM PHYS GRP, BOMBAY 400005, INDIA

来源：

STEROIDS | 1994年 / 59卷 / 06期

关键词：

STEROIDAL DOXYL NITROXIDE; 2'-(3-ALPHA-BENZYLOXY-24-NORCHOLAN-23-YL)-2'; 4'; 4'-TRIMETHYL-4'; 5'-DIHYDROOXAZOLINE-N-OXYL; SPIN LABEL; MODEL MEMBRANES; LOCALIZATION; PHASE TRANSITION; PERMEABILITY; MEPIVACAINE; XYLOCAINE; AZELAIC ACID;

D O I：

10.1016/0039-128X(94)90005-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new steroidal doxyl (4,4-dimethyloxazolidine-N-oxyl) nitroxide (SDN) viz. 2'-(3 alpha-benzyloxy-24-norcholan-2'-yl) -2',4',4'-trimethyl-4',5'-dihydrooxazoline-N-oxyl has been synthesized. This is expected to have higher mobility over other spin labels reported earlier. The localization of this spin probe in lipid bilayers has been determined using H-1 NMR and P-31 NMR techniques. The alterations induced by dugs in the membrane characteristics such as phase transition and permeability have been investigated using electron paramagnetic resonance (EPR) techniques. The results show the applicability of SDN as a potential spin probes in the study of biomembranes.

引用

页码：377 / 382

页数：6

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