A DRUG INCUBATION INFECTIVITY TEST (DIIT) FOR ASSESSING RESISTANCE IN TRYPANOSOMES

被引:24
作者
KAMINSKY, R [1 ]
GUMM, ID [1 ]
ZWEYGARTH, E [1 ]
CHUMA, F [1 ]
机构
[1] KENYA TRYPANOSOMIASIS RES INST,KIKUYU,KENYA
关键词
D O I
10.1016/0304-4017(90)90079-Q
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Blood stream forms of drug-resistant and sensitive Trypanosoma brucei brucei, Trypanosoma brucei evansi and Trypanosoma vivax were incubated in a liquid medium for 24 h at 37°C in the presence of various concentrations of diminazene aceturate (Berenil®) or isometamidium chloride (Samorin®), and assayed for infectivity in mice. Whereas the infectivity to mice of all Samorin-sensitive trypanosomes was decreased after incubation with 1 ng Samorin ml-1, the Samorin-resistant stocks remained infective for mice. Two of the Samorin-resistant stocks remained infective after incubation with Samorin concentrations of up to 50 ng ml-1. The infectivity of Berenil-resistant trypanosome stocks were also retained after incubation with drug concentrations (0.5 or 1.0 μg ml-1) which otherwise inhibited the infectivity of Berenil-sensitive trypanosome stocks. In addition, differences in infectivity were observed when Berenil-resistant and sensitive trypanosome stocks were incubated in medium supplemented with serum from goats previously treated with Berenil. Thus, drug-resistant and sensitive trypanosomes can be clearly distinguished using the drug incubation infectivity test. © 1990.
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页码:335 / 343
页数:9
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