A GROUP OF 3 FIBROBLAST SECRETED POLYPEPTIDES SUPPRESSED BY CELLULAR AGING AND INTERFERON-GAMMA

被引：3

作者：

ELEFTHERIOU, CS ^{[1
]}

TRAKAS, N ^{[1
]}

KOKLA, A ^{[1
]}

TZARTOS, SJ ^{[1
]}

机构：

[1] HELLEN INST PASTEUR,DEPT BIOCHEM,127 VAS SOFIAS AVE,GR-11521 ATHENS,GREECE

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1180卷 / 03期

关键词：

FIBROBLAST; SECRETED PROTEIN; AGING; INTERFERON-GAMMA;

D O I：

10.1016/0925-4439(93)90055-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The structural and quantitative characteristics of many fibroblast-secreted proteins are modified during the in vitro cellular ageing. Here we report that three polypeptides (80, 84 and 87 kDa) absent from late passage fibroblast cultures, are constitutively secreted from young (early passage) cultures of various fibroblast strains whereas they are suppressed by the action of interferon-gamma. The three polypeptides were isolated and monoclonal antibodies were produced against the 84 kDa polypeptide. Immunoprecipitation experiments showed that the three polypeptides share common epitopes. The 80 and 84 kDa polypeptides were studied further and proved to be glycosylated polypeptides exhibiting analogous CNBr digestion peptide maps and identity in their sequenced N-terminal segments.

引用

页码：304 / 312

页数：9

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