ISOLATION OF A BENZENE-TOLERANT BACTERIUM AND ITS HYDROCARBON DEGRADATION

被引:32
作者
MORIYA, K
HORIKOSHI, K
机构
[1] Deep star group, Japan Marine Science and Technology Center, Yokosuka, 237, 2-15, Natsushima-cho
来源
JOURNAL OF FERMENTATION AND BIOENGINEERING | 1993年 / 76卷 / 03期
关键词
D O I
10.1016/0922-338X(93)90002-P
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
After treatment of deep-sea sediment samples (collected from a depth of 1,945-m in Suruga Bay) with 20 v/v% benzene followed by 50 v/v% kerosene, fifty-six halotolerant bacteria were isolated as candidate hydrocarbon degraders that also possessed the characteristic of benzene-tolerance. One of the isolates showed halotolerant growth and tolerance to various kinds of organic solvents (OSs) such as 5 v/v% benzene, 10 v/v% toluene and 10 v/v% p-xylene. From these characteristics, in addition to its morphological and biochemical characteristics, it seemed to be a variant strain of Flavobacterium sp. and was tentatively named strain no. DS-711. The correlation between log p, used as a parameter for the degree of toxicity of the OSs, and growth on the OSs was further examined using the benzene-tolerant strain DS-711 and the toluene-tolerant Pseudomonas putida strain IH-2000. The results indicated that the mechanism of OS tolerance of DS-711 was different from that of P. putida strain IH-2000. Furthermore, a hydrocarbon degradation experiment indicated that the benzene-tolerant strain DS-711 had a greater n-alkane degrading ability compared to that of our selected control strains, the OS-sensitive Alteromonas sp. strain no. DS-201 and the toluene-tolerant P. putida strain IH-2000. With stepwise addition of concentrated cell suspension, strain DS-711 could degrade more than 90% of n-alkanes in kerosene.
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页码:168 / 173
页数:6
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