MONOCLONAL-ANTIBODIES TO CONSERVED REGIONS OF THE MAJOR CORE PROTEIN (GAG24) OF HIV-1 AND HIV-2

被引：10

作者：

CARPIO, E

DUARTE, C

HINKULA, J

BROLIDEN, PA

ROSEN, J

CAMPAL, A

GAVILONDO, J

WAHREN, B

JONDAL, M

机构：

[1] KAROLINSKA INST, DEPT IMMUNOL, S-10401 STOCKHOLM 60, SWEDEN

[2] CTR GENET ENGN & BIOTECHNOL, DIV HYBRIDOMA, HAVANA, CUBA

[3] NATL BACTERIOL LAB, DEPT VIROL, S-10521 STOCKHOLM, SWEDEN

[4] RW JOHNSON PHARMACEUT RES INST, SAN DIEGO, CA 92121 USA

来源：

AIDS RESEARCH AND HUMAN RETROVIRUSES | 1991年 / 7卷 / 01期

关键词：

D O I：

10.1089/aid.1991.7.97

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Five mouse monoclonal antibodies were raised against a recombinant protein comprising the complete sequence of gag24 protein from HTLV-III(B). All monoclonal antibodies recognized the native protein in enzyme-linked immunosorbant assay (ELISA) and Western blots. All monoclonal antibodies also cross-reacted with an human immunodeficiency virus type 2 (HIV-2) strain in western blots, whereas only one antibody detected HIV-2 p25 in ELISA. By using synthetic peptides, cross-reacting epitopes were mapped and three regions were defined. The conserved immunogenic sites were located in the carboxyterminal region of the protein. Inhibition experiments with human sera showed that this region is also immunogenic in humans.

引用

页码：97 / 101

页数：5

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