CONFORMATIONAL BEHAVIOR OF POLY(BETA-BENZYL L-ASPARTATE) IN THE SOLID-STATE AS STUDIED BY VARIABLE-TEMPERATURE C-13 CROSS-POLARIZATION MAGIC ANGLE SPINNING NUCLEAR-MAGNETIC-RESONANCE SPECTROSCOPY

被引：28

作者：

AKIEDA, T ^{[1
]}

MIMURA, H ^{[1
]}

KUROKI, S ^{[1
]}

KUROSU, H ^{[1
]}

ANDO, I ^{[1
]}

机构：

[1] TOKYO INST TECHNOL,DEPT POLYMER CHEM,MEGURO KU,TOKYO 152,JAPAN

来源：

MACROMOLECULES | 1992年 / 25卷 / 21期

关键词：

D O I：

10.1021/ma00047a035

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Carbon-13 cross-polarization/magic angle spinning nuclear magnetic resonance experiments are carried out for poly(beta-benzyl L-aspartate)(PBLA) over a wide range of temperatures, in order to elucidate the stability of the conformation in the solid state. From these experimental results, we obtained the following results on the thermal stability of the main chain. The main chain of PBLA takes on a right-handed a-helical conformation (alpha(R)-helix) within the temperature range from room temperature (23-degrees-C) to 117-degrees-C, and the main-chain conformation predominantly changes at about 128-degrees-C from an alpha(R)-helix form to a left-handed omega-helical form (omega(L)-helix), although minor transition to the beta-sheet form occurs. In the temperature range from 128 to 139-degrees-C a mixture of three types of conformations, the alpha(R)-helix, omega(L)-helix, and beta-sheet forms, coexist. At 150-degrees-C the alpha(R)-helix form completely transforms to the omega(L)-helix or beta-sheet form, and in the temperature range from 150 to 161-degrees-C a mixture of both the forms exists. At 173-degrees-C the maim-chain conformation completely becomes the beta-sheet conformation.

引用

页码：5794 / 5797

页数：4

共 20 条

[1] CONFORMATION-DEPENDENT C-13 NMR CHEMICAL-SHIFTS OF POLY(L-ALANINE) IN THE SOLID-STATE - FPT INDO CALCULATION OF N-ACETYL-N'-METHYL-L-ALANINE AMIDE AS A MODEL-COMPOUND OF POLY(L-ALANINE) [J].

ANDO, I ;

SAITO, H ;

TABETA, R ;

SHOJI, A ;

OZAKI, T .

MACROMOLECULES, 1984, 17 (03) :457-461

[2] STRUCTURE AND MOLECULAR PACKING OF OMEGA FORM OF POLY (BETA-BENZYL L-ASPARTATE) ELECTRON-DIFFRACTION STUDIES [J].

BALDWIN, JP ;

BRADBURY, EM ;

MCLUCKIE, IF ;

STEPHENS, RM .

MACROMOLECULES, 1973, 6 (01) :83-91

[3] STRUCTURE OF OMEGA-FORM OF POLY-BETA-BENZYL-L-ASPARTATE [J].

BRADBURY, EM ;

HANBY, WE ;

BROWN, L ;

FRASER, RDB ;

DOWNIE, AR ;

ELLIOTT, A .

JOURNAL OF MOLECULAR BIOLOGY, 1962, 5 (02) :230-&

[4] TOTAL SUPPRESSION OF SIDEBANDS IN CPMAS C-13 NMR [J].

DIXON, WT ;

SCHAEFER, J ;

SEFCIK, MD ;

STEJSKAL, EO ;

MCKAY, RA .

JOURNAL OF MAGNETIC RESONANCE, 1982, 49 (02) :341-345

[5] SECONDARY STRUCTURE OF PEPTIDES .3. C-13 NMR CROSS POLARIZATION MAGIC ANGLE SPINNING SPECTROSCOPIC CHARACTERIZATION OF SOLID POLYPEPTIDES [J].

KRICHELDORF, HR ;

MULLER, D .

MACROMOLECULES, 1983, 16 (04) :615-623

[6]

KRICHELDORF HR, 1983, POLYM BULL, V9, P284

[7] SECONDARY STRUCTURE OF PEPTIDES .4. C-13-NMR CP/MAS INVESTIGATION OF SOLID OLIGO(L-ALANINE) AND POLY(L-ALANINE) [J].

KRICHELDORF, HR ;

MUTTER, M ;

MASER, F ;

MULLER, D ;

FORSTER, H .

BIOPOLYMERS, 1983, 22 (05) :1357-1372

[8] A HIGH-RESOLUTION C-13-NMR STUDY OF COLLAGENLIKE POLYPEPTIDES AND COLLAGEN FIBRILS IN SOLID-STATE STUDIED BY THE CROSS-POLARIZATION MAGIC ANGLE-SPINNING METHOD - MANIFESTATION OF CONFORMATION-DEPENDENT C-13 CHEMICAL-SHIFTS AND APPLICATION TO CONFORMATIONAL CHARACTERIZATION [J].

SAITO, H ;

TABETA, R ;

SHOJI, A ;

OZAKI, T ;

ANDO, I ;

MIYATA, T .

BIOPOLYMERS, 1984, 23 (11) :2279-2297

[9] HIGH-RESOLUTION C-13 NMR-STUDY OF SILK FIBROIN IN THE SOLID-STATE BY THE CROSS-POLARIZATION MAGIC ANGLE SPINNING METHOD - CONFORMATIONAL CHARACTERIZATION OF SILK-I AND SILK-II TYPE FORMS OF BOMBYX-MORI FIBROIN BY THE CONFORMATION-DEPENDENT C-13 CHEMICAL-SHIFTS [J].

SAITO, H ;

TABETA, R ;

ASAKURA, T ;

IWANAGA, Y ;

SHOJI, A ;

OZAKI, T ;

ANDO, I .

MACROMOLECULES, 1984, 17 (07) :1405-1412

[10] CONFORMATIONAL CHARACTERIZATION OF POLYPEPTIDES IN THE SOLID-STATE AS VIEWED FROM THE CONFORMATION-DEPENDENT C-13 CHEMICAL-SHIFTS DETERMINED BY THE C-13 CROSS POLARIZATION MAGIC ANGLE SPINNING METHOD - OLIGO(L-ALANINE), POLY(L-ALANINE), CO-POLYMERS OF L-ALANINE AND D-ALANINE, AND CO-POLYMERS OF L-ALANINE WITH N-METHYL-L-ALANINE OR N-BENZYL-L-ALANINEGL [J].

SAITO, H ;

TABETA, R ;

SHOJI, A ;

OZAKI, T ;

ANDO, I .

MACROMOLECULES, 1983, 16 (07) :1050-1057

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