IN-SITU QUANTIFICATION OF STAGE-SPECIFIC APOPTOSIS IN THE RAT SEMINIFEROUS EPITHELIUM - EFFECTS OF SHORT-TERM EXPERIMENTAL CRYPTORCHIDISM

被引:66
作者
HENRIKSEN, K
HAKOVIRTA, H
PARVINEN, M
机构
[1] Department of Anatomy, Institute of Biomedicine, University of Turku
来源
INTERNATIONAL JOURNAL OF ANDROLOGY | 1995年 / 18卷 / 05期
关键词
APOPTOSIS; CRYPTORCHIDISM; GERM CELLS; RAT; TESTIS;
D O I
10.1111/ijan.1995.18.5.256
中图分类号
R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
摘要
Two techniques have been combined for quantification of apoptotic germ cells in defined stages of the cycle of the seminiferous epithelium: the improved transillumination method and nonradioactive in-situ end-labelling of DNA (ISEL). Segments of rat seminiferous tubules were squashed between a microscope slide and coverslip, and the stage identified under a phase-contrast microscope. After fixation, apoptotic cells were detected by ISEL and scored per 1 mm tubule. In the normal testis apoptotic cells were found in all stages, the highest frequency occurring in stages XII-XIV (19 cells/mm). In short-term (24 and 48 h) experimentally cryptorchid testes, a significant increase in number of apoptotic germ cells was evident in all stages, except for VI and VIII. Apoptosis of germ cells was confirmed by electrophoresis of radioactively labelled DNA from stages VII-VIII and XIII-I. It is proposed that apoptosis is a means of eliminating the most sensitive germ cells after short-term experimental cryptorchidism.
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页码:256 / 262
页数:7
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