RESEARCH NOTE - THE EVALUATION OF CHICKEN SPERMATOZOA USING FLUORESCENT STAINING IN A 96-WELL FORMAT

被引：12

作者：

BAYYARI, GR

COOK, JR

HARRIS, GC

MACY, LB

SLAVIK, MF

SKEELES, IK

机构：

[1] Department of Animal and Poultry Sciences, University of Arkansas, Fayetteville

来源：

POULTRY SCIENCE | 1990年 / 69卷 / 09期

关键词：

D O I：

10.3382/ps.0691602

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

An efficient method for the evaluation of spermatozoa using the fluorescent stains carboxyfluorescein diacetate and propidium iodide and an automated fluorescence concentration analyzer was adapted for chicken semen. Arbitrary fluorescence units representing either live or dead spermatozoa were strongly correlated with percentage of added dead spermatozoa and with the direct fluorescent microscope counts of live, dead, and damaged cells.

引用

页码：1602 / 1605

页数：4

共 12 条

[1]

ALMLID T, 1988, J ANIM SCI, V66, P2899

[2] FLUOROMETRIC-DETERMINATION OF AVIAN SPERM VIABILITY AND CONCENTRATION [J].