MOLECULAR-CLONING OF A NOVEL PHYTOCHROME GENE OF THE MOSS CERATODON-PURPUREUS WHICH ENCODES A PUTATIVE LIGHT-REGULATED PROTEIN-KINASE

被引:82
作者
THUMMLER, F [1 ]
DUFNER, M [1 ]
KREISL, P [1 ]
DITTRICH, P [1 ]
机构
[1] MARTINSRIEDER INST PROTEINSEQUENZEN, MPI BIOCHEM, W-8033 MARTINSRIED, GERMANY
关键词
MOSS; PHOTORECEPTOR; PHYTOCHROME; PROTEIN KINASE; PROTEIN-TYROSINE KINASE; SIGNAL TRANSDUCTION;
D O I
10.1007/BF00028888
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The phytochrome gene (phyCer) of the moss Ceratodon purpureus was isolated and characterized. phyCer is composed of three coding exons: exon I of 2035 bp, exon II of 300 bp and exon III of 1574 bp. The deduced polypeptide encoded by exon I and II exhibits substantial sequence homology to the conserved NH2-terminal chromophore domain of known phytochromes. In contrast, the COOH-terminal polypeptide encoded by exon III shows no sequence homology to any phytochrome molecule. phyCer most likely represents a single-copy gene and is expressed in a light-independent manner. From the DNA sequence analysis it can be deduced that the PhyCer polypeptide is composed of 1303 amino acids (including the starting Met) which predicts a molecular mass for PhyCer of 145 kDa. The polypeptide encoded in exon III exhibits striking homology within the 300 carboxy-terminal amino acids to the catalytic domain of protein kinases. The carboxy terminus of PhyCer was found to be most homologous to protein-tyrosine kinases of Dictyostelium discoideum and to the products of retroviral oncogenes which belong to the Raf-Mos serine/threonine kinase family. From the hydropathy profile PhyCer appears to be a soluble protein. The predicted structure suggests that PhyCer represents a soluble light-sensor protein kinase which is linked with a cellular phosphorylating cascade.
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页码:1003 / 1017
页数:15
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